Brilliant Violet 785™ anti-human CD63 Antibody

Pricing & Availability
Clone
H5C6 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
LIMP, LAMP-3, Melanoma-associated antigen (ME491), Pltgp40, gp55
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
H5C6_BV785_CD63_Antibody_080619_updated.png
Thrombin-activated human peripheral blood platelets were stained with CD63 (clone H5C6) Brilliant Violet 785™ (filled histogram) or mouse IgG1, κ Brilliant Violet 785™ isotype control (open histogram).
  • H5C6_BV785_CD63_Antibody_080619_updated.png
    Thrombin-activated human peripheral blood platelets were stained with CD63 (clone H5C6) Brilliant Violet 785™ (filled histogram) or mouse IgG1, κ Brilliant Violet 785™ isotype control (open histogram).
Compare all formats See Brilliant Violet 785™ spectral data
Cat # Size Price Quantity Check Availability Save
353043 25 tests 156,00€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
353044 100 tests 324,00€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD63 is a 53 kD type III lysosomal glycoprotein also known as LIMP, LAMP-3, gp55, and melanoma-associated antigen (ME491). CD63 is a member of the tetraspan transmembrane superfamily (TM4SF) protein expressed on activated platelets, monocytes/macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle cells. CD63 may be involved in platelet activation and is thought to function as a transmembrane adaptor protein. CD63 has been shown to associate with CD9, CD81, VLA-3, and VLA-6.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
T cell line HPB-ALL
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1, immunofluorescence2, and immunoprecipitation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Hildreth JE, et al. 1991. Blood 77:121. (IP, WB)
  2. Beatty WL, et al. 2006. J. Cell Sci. 119:350. (IF)
RRID
AB_2814288 (BioLegend Cat. No. 353043)
AB_2814289 (BioLegend Cat. No. 353044)

Antigen Details

Structure
Tetraspan transmembrane superfamily (TM4SF), type III lysosomal glycoprotein, 53 kD
Distribution

Activated platelets, monocytes, macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle

Function
Platelet activation
Interaction
CD9, CD81, VLA-3, and VLA-6
Cell Type
B cells, Endothelial cells, Fibroblasts, Macrophages, Monocytes, Osteoclasts, Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Azorsa DO, et al. 1991. Blood 78:280.
  2. Kishimoto T, et al. Eds. 1997. Leukocyte Typing V1. Oxford University Press New York.
  3. Hildreth JE, et al. 1991. Blood 77:121.
  4. Anzai N, et al. 2002. Blood 99:4413.
Gene ID
967 View all products for this Gene ID
UniProt
View information about CD63 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08.06.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • PE anti-human CD63

  • FITC anti-human CD63

  • Pacific Blue™ anti-human CD63

  • APC anti-human CD63

  • PE/Cyanine7 anti-human CD63

  • Alexa Fluor® 647 anti-human CD63

  • Biotin anti-human CD63

  • PerCP/Cyanine5.5 anti-human CD63

  • PE/Dazzle™ 594 anti-human CD63

  • Brilliant Violet 421™ anti-human CD63

  • Brilliant Violet 650™ anti-human CD63

  • APC/Fire™ 750 anti-human CD63

  • Alexa Fluor® 700 anti-human CD63

  • Brilliant Violet 510™ anti-human CD63

  • Alexa Fluor® 594 anti-human CD63

  • TotalSeq™-A0404 anti-human CD63

  • Alexa Fluor® 488 anti-human CD63

  • Ultra-LEAF™ Purified anti-human CD63

  • Brilliant Violet 711™ anti-human CD63

  • Brilliant Violet 785™ anti-human CD63

  • APC/Cyanine7 anti-human CD63

  • TotalSeq™-B0404 anti-human CD63

  • TotalSeq™-C0404 anti-human CD63

  • Brilliant Violet 605™ anti-human CD63 Antibody

  • PE/Cyanine5 anti-human CD63 Antibody

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account