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Brilliant Violet 711™ anti-human CD146 Antibody

Pricing & Availability
Clone
P1H12 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
Muc-18, MCAM, Mel-CAM, S-endo
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P1H12_BV711_CD146_Antibody_032819
Human cervical cancer cell line, HeLa, was stained with CD146 (clone P1H12) Brilliant Violet 711™ (filled histogram) or mouse IgG1, κ Brilliant Violet 711™ isotype control (open histogram).
  • P1H12_BV711_CD146_Antibody_032819
    Human cervical cancer cell line, HeLa, was stained with CD146 (clone P1H12) Brilliant Violet 711™ (filled histogram) or mouse IgG1, κ Brilliant Violet 711™ isotype control (open histogram).
Compare all formats See Brilliant Violet 711™ spectral data
Cat # Size Price Quantity Check Availability Save
361031 25 tests 172€
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361032 100 tests 366€
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Description

CD146 is a 118 kD integral transmembrane glycoprotein that is also known as MUC18, S-Endo, MCAM, and Mel-CAM (melanoma cell adhesion molecule). It belongs to the immunoglobulin superfamily. CD146 is expressed on melanoma cells, epithelial cells, endothelial cells, fibroblasts, activated T cells, multipotent mesenchymal stromal cells, and activated keratinocytes. CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration. The ligand of CD146 remains to be identified.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Mouse, Dog, Rabbit
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Cultured human umbilical cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats of this clone) include: Western blotting3 and IHC1,5. 

Clone P1H12 cross-reacts to Dog2, 5.

Application References

(PubMed link indicates BioLegend citation)
  1. Solovey A, et al. 1997. N. Engl. J. Med. 337:1584. (FC, IHC)
  2. Lamerato-Kozicki AR, et al. 2006. Exp. Hematol. 34:870. (FC)
  3. Balint K, et al. 2005. J. Clin. Invest. 115:3166. (WB)
  4. Neskey DM, et al. 2008. J. Exp. Clin. Cancer Res. 27:61. (ELISA)
  5. Kamstock D, et al. 2006. Cancer Gene Therap. 13:306. (IHC)
RRID
AB_2800997 (BioLegend Cat. No. 361031)
AB_2800998 (BioLegend Cat. No. 361032)

Antigen Details

Structure
118 kD integral glycoprotein, Ig superfamily
Distribution

Melanoma cells, epithelial cells, endothelial cells, activated T cells, multipotent mesenchymal stromal cells

Function
Adhesion, monocytes transendothelial migration
Cell Type
Endothelial cells, Epithelial cells, Mesenchymal cells, Mesenchymal Stem Cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Pickl WF, et al. 1997. J. Immunol. 158:2107.
2. Weninger W, et al. 2000. J. Invest. Dermatol. 115:219.
3. Sorrentino A, et al. 2008. Exp. Hematol. 36:1035.
4. Bardin N, et al. 2009. Arterioscler. Thromb. Vasc. Biol. 29:746.

Gene ID
4162 View all products for this Gene ID
UniProt
View information about CD146 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12.16.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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