Brilliant Violet 650™ anti-mouse/rat XCR1 Antibody

Pricing & Availability
ZET (See other available formats)
Regulatory Status
Other Names
GPR5, CCXCR1, mXCR1, Lymphotactin receptor, SCM1 receptor, XC chemokine receptor 1
Mouse IgG2b, κ
Ave. Rating
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Product Citations
Cells from collagenase-digested C57BL/6 mouse spleen were stained with CD11c APC/Cy7, CD8α FITC, and XCR1 (clone ZET) Brilliant Violet 650™ (top) or mouse IgG2b, κ Brilliant Violet 650™ isotype control (bottom). Data shown was gated on the CD11c+ population.
  • ZET_BV650_XCR1_Antibody_FC_1_102715
    Cells from collagenase-digested C57BL/6 mouse spleen were stained with CD11c APC/Cy7, CD8α FITC, and XCR1 (clone ZET) Brilliant Violet 650™ (top) or mouse IgG2b, κ Brilliant Violet 650™ isotype control (bottom). Data shown was gated on the CD11c+ population.
  • ZET_BV650_XCR1_Antibody_FC_2_102715
See Brilliant Violet 650™ spectral data
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148220 50 µg 228,00€
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XCR1, also known as GPR5 or CCXCR1, is a 38 kD, G-protein coupled, seven transmembrane receptor, and the only member of the "C" chemokine receptor family. XCR1 mediates chemotaxis of XCL1 and XCL2 (lymphotactin-1 and -2), and defines a subset of CD8α+ conventional dendritic cells capable of antigen cross-presentation.

Product Details
Technical Data Sheet (pdf)

Product Details

Mouse, Rat
Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissues2.

Application References

(PubMed link indicates BioLegend citation)
  1. Gurka S, et al. 2015. Front Immunol. 6:35. (FC)
  2. Kitazawa Y, et al. 2019. Front Immunol. 10:1195. (FC, IHC-F)
Product Citations
  1. Hayashi K, et al. 2020. Nat Commun. 4.832638889. PubMed
  2. Silva-Cayetano A, et al. 2020. Med. 2(3):243-262.e8. PubMed
  3. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  4. Marangoni F, et al. 2021. Cell. . PubMed
  5. Chopin M et al. 2018. Immunity. 50(1):77-90 . PubMed
  6. Bosteels C, et al. 2020. Immunity. 52(6):1039-1056.e9.. PubMed
  7. Yu YR, et al. 2020. STAR Protocols. 11(3):e0150606. PubMed
  8. Leruste A, et al. 2020. Cancer Cell. 36(6):597-612.e8.. PubMed
  9. Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
  10. Remmerie A, et al. 2020. Immunity. 53:641. PubMed
  11. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  12. Liu Z, et al. 2020. STAR Protoc. 1:100029. PubMed
AB_2566410 (BioLegend Cat. No. 148220)

Antigen Details

Only member of the "C" chemokine receptor family, seven transmembrane receptor, coupled to G protein, 38 kD.

Subset of CD8α+ conventional dendritic cells.

Mediates chemotaxis to XCL1, defines a population of CD8α+ dendritic cells capable of antigen cross-presentation to CD8+ T cells.
XCL1 and XCL2.
Cell Type
Dendritic cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Yamazaki C, et al. 2013. J. Immunol. 190:6071.
2. Shimizu K, et al. 2013. J. Immunol. 190:5609.
3. Bachem A, et al. 2012. Front. Immunol. 3:214.
4. Crozat K, et al. 2011. J. Immunol. 187:4411.

Gene ID
23832 View all products for this Gene ID 301086 View all products for this Gene ID
View information about XCR1 on

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Go To Top Version: 1    Revision Date: 10.28.2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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