- YTS156.7.7 (See other available formats)
- Regulatory Status
- Other Names
- Lyt-3, Ly-3
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
|Cat #||Size||Price||Quantity Check Availability||Save|
CD8b is the 32 kD β chain of CD8, also known as Lyt-3 or Ly-3. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Mouse thymocytes
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen tissue sections.
(PubMed link indicates BioLegend citation)
- McNical AM, et al. 2007. Eur. J. Immunol. 37:1634.
- Product Citations
AB_2800621 (BioLegend Cat. No. 126631)
- Ig superfamily, CD8β chain, 34 kD
Majority of thymocytes, cytotoxic T cell subsets
- Co-receptor of TCR
- MHC class I
- Cell Type
- T cells, Thymocytes, Tregs
- Biology Area
- Molecular Family
- CD Molecules
- Antigen References
1.Barclay A, et al. 1997. The Leukocyte antigen Facts Book Academic Press.
2. Zamoyska R. 1994. Immunity 1:243-246.
3. Ellmeier W, et al. 1999. Annu. Rev Immunol 17:523.
4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503.
- Gene ID
- 12526 View all products for this Gene ID
- View information about CD8b on UniProt.org
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD8b
Biotin anti-mouse CD8b
FITC anti-mouse CD8b
PE anti-mouse CD8b
PerCP/Cyanine5.5 anti-mouse CD8b
Alexa Fluor® 647 anti-mouse CD8b
APC anti-mouse CD8b
PE/Cyanine7 anti-mouse CD8b
Alexa Fluor® 700 anti-mouse CD8b
APC/Cyanine7 anti-mouse CD8b
PE/Dazzle™ 594 anti-mouse CD8b
TotalSeq™-A0230 anti-mouse CD8b (Ly-3)
Brilliant Violet 510™ anti-mouse CD8b (Ly-3)
Brilliant Violet 711™ anti-mouse CD8b (Ly-3)
Alexa Fluor® 488 anti-mouse CD8b (Ly-3)
APC/Fire™ 750 anti-mouse CD8b (Ly-3)
Brilliant Violet 421™ anti-mouse CD8b (Ly-3)
Alexa Fluor® 594 anti-mouse CD8b (Ly-3)
TotalSeq™-B0230 anti-mouse CD8b (Ly-3)
TotalSeq™-C0230 anti-mouse CD8b (Ly-3)