- SJ25C1 (See other available formats)
- Regulatory Status
- Other Names
- CD19 Molecule, CD19 Antigen, Differentiation Antigen CD19, B-Lymphocytes Surface Antigen B4, T-cell Surface Antigen Leu-12, CVID3, B4, B-lymphocyte Antigen CD19
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- NALM1 + NALM16 cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
IHC-F - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
Clone SJ25C1 partially blocks anti-human CD19 clones HIB19 and 4G7 staining based on in-house testing
- Product Citations
AB_2564226 (BioLegend Cat. No. 363017)
AB_2564227 (BioLegend Cat. No. 363018)
- Ig superfamily, type I transmembrane glycoprotein, 95 kD.
B lineage (except plasma cells), follicular dendritic cells.
- B cell activation and differentiation.
- Forms a complex with CD21, CD81 and CD225 in the membrane of mature B cells and interacts with VAV and LYN. It also interacts with GRAB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378 and with PLCG2 when phosphorylated on Tyr-409.
- Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR co-receptor.
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.
- Gene ID
- 930 View all products for this Gene ID
- View information about CD19 on UniProt.org
- What is the F/P ratio range of our BV421™ format antibody reagents?
It is lot-specific. On average it ranges between 2-4.
|Brilliant Violet 421™ anti-human CD19||SJ25C1||FC, IHC-F|
|Purified anti-human CD19||SJ25C1||FC, IHC-F|
|PE anti-human CD19||SJ25C1||FC|
|APC anti-human CD19||SJ25C1||FC|
|FITC anti-human CD19||SJ25C1||FC|
|APC/Cyanine7 anti-human CD19||SJ25C1||FC|
|PE/Cyanine7 anti-human CD19||SJ25C1||FC|
|PerCP anti-human CD19||SJ25C1||FC|
|PerCP/Cyanine5.5 anti-human CD19||SJ25C1||FC|
|Brilliant Violet 510™ anti-human CD19||SJ25C1||FC|
|Brilliant Violet 711™ anti-human CD19||SJ25C1||FC|
|Brilliant Violet 605™ anti-human CD19||SJ25C1||FC|
|Brilliant Violet 650™ anti-human CD19||SJ25C1||FC|
|Brilliant Violet 785™ anti-human CD19||SJ25C1||FC|
|APC/Fire™ 750 anti-human CD19||SJ25C1||FC|
|PE/Dazzle™ 594 anti-human CD19||SJ25C1||FC|
|Alexa Fluor® 700 anti-human CD19||SJ25C1||FC|
|Pacific Blue™ anti-human CD19||SJ25C1||FC|
|Alexa Fluor® 488 anti-human CD19||SJ25C1||FC|
|Alexa Fluor® 647 anti-human CD19||SJ25C1||FC, IHC-F|
|PE/Cyanine5 anti-human CD19||SJ25C1||FC|
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Brilliant Violet 421™ anti-human CD19
Purified anti-human CD19
PE anti-human CD19
APC anti-human CD19
FITC anti-human CD19
APC/Cyanine7 anti-human CD19
PE/Cyanine7 anti-human CD19
PerCP anti-human CD19
PerCP/Cyanine5.5 anti-human CD19
Brilliant Violet 510™ anti-human CD19
Brilliant Violet 711™ anti-human CD19
Brilliant Violet 605™ anti-human CD19
Brilliant Violet 650™ anti-human CD19
Brilliant Violet 785™ anti-human CD19
APC/Fire™ 750 anti-human CD19
PE/Dazzle™ 594 anti-human CD19
Alexa Fluor® 700 anti-human CD19
Pacific Blue™ anti-human CD19
Alexa Fluor® 488 anti-human CD19
Alexa Fluor® 647 anti-human CD19
PE/Cyanine5 anti-human CD19