Biotin anti-Synapsin I/II/III Antibody

Pricing & Availability
Clone
A17080A (See other available formats)
Regulatory Status
RUO
Other Names
SYN, Synapsin, Synapsin I, Synapsin II, Synapsin III
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1-A17080A_Biotin_Synapsin_Antibody_WB_3_020419.png
Western blot of Biotin anti-Synapsin I/II/III antibody (clone A17080A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of rat brain lysate; The blot was incubated with 0.01 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Streptavidin (Cat. No. 405210). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • 1-A17080A_Biotin_Synapsin_Antibody_WB_3_020419.png
    Western blot of Biotin anti-Synapsin I/II/III antibody (clone A17080A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of rat brain lysate; The blot was incubated with 0.01 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Streptavidin (Cat. No. 405210). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • 2-A17080A_Biotin_Synapsin_Antibody_IHC_1_020419.png
    IHC staining of Biotin anti-Synapsin I/II/III antibody (clone A17080A) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 Streptavidin (Cat. No. 405240) for one hour at room temperature. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 100 µm
  • 3-A17080A_Biotin_Synapsin_Antibody_IHC_2_020419.png
    IHC staining of Biotin anti-Synapsin I/II/III antibody (clone A17080A) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 Streptavidin (Cat. No. 405240) for one hour at room temperature. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 100 µm
Compare all formats
Cat # Size Price Quantity Check Availability Save
853705 25 µg 118€
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853706 100 µg 264€
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Description

Synapsins are a family of proteins involved in neurotransmitter release at the chemical synapses. Synapsins anchor synaptic vesicles to the cytoskeletal structures at the presynaptic terminal. Upon receiving an action potential, phosphorylation of synapsins, possibly by PKA, leads to the release of synaptic vesicles from cytoskeletal structures. This allows membrane docking and fusion of synaptic vesicles with the presynaptic membrane and neurotransmitter release. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein corresponding to amino acids 362-450 of human Synapsin I
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

WB - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.001 - 0.01 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 0.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Yasom S, et al. 2022. FASEB Bioadv. 4:408. PubMed
RRID
AB_2801203 (BioLegend Cat. No. 853705)
AB_2801204 (BioLegend Cat. No. 853706)

Antigen Details

Structure
Synapsin I is a 704 amino acid protein with an apparent molecular mass of ~74 kD. Synapsin II is a 582 amino acid protein with an apparent molecular mass of ~63 kD. Synapsin III is a 580 amino acid protein with an apparent molecular mass of ~63 kD.
Distribution

Tissue distribution: Mainly in the central nervous system. Also expressed in testis.
Cellular distribution: Cytosol, nucleus, and plasma membrane.

Function
Synapsins are involved in the regulation of synaptic vesicle fusion and neurotransmitter release at the presynaptic terminals.
Interaction
CAPON (Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein), PKA
Cell Type
Neurons
Biology Area
Cell Biology, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Presynaptic proteins, Synaptic Vesicle Trafficking/Endocytosis
Antigen References
  1. Greengard P, et al. 1993. Science. 259(5096):780
  2. Milovanovic D, et al. 2017. Neuron. 93(5):995
  3. Evergren E, et al. 2007. J. Neurosci. Res. 85(12):2648
Gene ID
6853 View all products for this Gene ID 6854 View all products for this Gene ID 8224 View all products for this Gene ID
UniProt
View information about Synapsin I/II/III on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 3    Revision Date: 10.11.2019

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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  • Alexa Fluor® 594 anti-Synapsin I/II/III

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  • Alexa Fluor® 647 anti-Synapsin I/II/III

  • Spark YG™ 570 anti-Synapsin I/II/III Antibody

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