- Clone
- 145-2C11; RB6-8C5; RA3-6B2; Ter-119; M1/70;
- Other Names
- Lin-1
- Isotype
- Armenian Hamster IgG, Rat IgG2b, Rat IgG2a
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
C57BL/6 mouse bone marrow cells were stained with Biotin Lineage Cocktail, followed by Sav-FITC and CD117 APC.
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 133307 | 500 tests | 220€ | ||||
The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. The Biotin anti-mouse Lineage Panel is designed for the flow cytometric identification and immune-enrichment of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3, clone 145-2C11; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5; anti-mouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3-6B2; anti-mouse TER-119/Erythroid cells, clone Ter-119.
Materials provided (one vial each):
Part No. Description
79748 anti-mouse TER-119/Erythroid Cells
79749 anti-mouse CD11b
79750 anti-mouse Ly-6G/Ly-6C (Gr-1)
79751 anti-mouse CD3e
79752 anti-mouse CD45R/B220
Materials not provided:
1. Isotype Controls
2. Cell Staining Buffer (Cat. No. 420201)
Product Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solutions should be stored undiluted at 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
Magnetic particle separation - Verified - Recommended Usage
-
Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 2 µl of each antibody per million cells or 2 µl of each antibody per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
Antigen Details
- Distribution
-
T cells, B cells, monocytes/macrophages, granulocytes, NK cells, and erythrocytes
- Cell Type
- B cells, Erythrocytes, Granulocytes, Macrophages, Monocytes, NK cells, T cells
- Biology Area
- Immunology
- Antigen References
-
1. Morrison SJ, et al. 1997. Development 124:1929.
2. Okada S, et al. 1992. Blood 80:3044.
3. Spangrude GJ, et al. 1988. Science 241:58.
4. Spangrude GJ, et al. 1990. Exp Hematol. 18:920.
5. Tan SL, et al. 2006. J. Immunol. 176:2872. - Gene ID
- 104231 View all products for this Gene ID
- UniProt
- View information about Lineage on UniProt.org
Related FAQs
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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