- BVD6-24G2 (See other available formats)
- Regulatory Status
- Other Names
- Interleukin-4, Ia inducing factor (IaIF), B cell stimulating factor-1 (BSF-1), Hodgkin's cell growth factor (HCGF), Mast cell growth factor-2 (MCGF-2), Macrophage fusion factor (MFF), T cell growth factor-2 (TCGF-2)
- Rat IgG1, κ
- Ave. Rating
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- Product Citations
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IL-4 is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. IL-4 is a potent lymphoid cell growth factor which stimulates the growth and activation of certain B cells and T cells. IL-4 is important for regulation of T helper subset development. The BVD6-24G2 antibody reacts with mouse interleukin-4 (IL-4). The BVD6-24G2 antibody can not neutralize the bioactivity of natural or recombinant IL-4.Product Details
- Antibody Type
- Host Species
- E. coli-expressed, recombinant mouse IL-4
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
ELISA Detection, ELISPOT Detection
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-4 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA Detection1-3,5,7,8 or ELISPOT Detection4: The biotinylated BVD6-24G2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified 11B11 antibody (Cat. No. 504102/504108) as capture antibody and recombinant mouse IL-4 (Cat. No. 563201) as the standard.
Additional reported applications (for the relevant formats) include: In Vivo Capture6.
Note: For testing mouse IL-4 in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 431101 to 431106) are specially developed and recommended.
(PubMed link indicates BioLegend citation)
- Abrams J, et al. 1992. Immunol. Rev. 127:5.
- Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.20.
- Sander B, et al. 1993. J. Immunol. Meth. 166:201.
- Fujihashi K, et al. 1991. J. Immunol. Meth. 160:181.
- Sander B, et al. 1991. Immunol. Rev. 119:65.
- Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons New York. Unit 6.28.
- Dzhagalov I, et al. 2007. J. Immunol. 178:2113.
- Xu G, et al. 2007. J. Immunol. 179:5358.
- Paveley RA, et al. 2011. Int J Parasitol. 41:1335. PubMed
- Product Citations
AB_315323 (BioLegend Cat. No. 504201)
AB_315324 (BioLegend Cat. No. 504202)
- Cytokine; 15-19 kD (Mammalian)
- Differentiation of naïve CD4+ T cells to the TH2 type, proliferation/differentiation of activated B cells, expression of class II MHC antigens, and of low affinity IgE receptors in resting B cells
- Cell Sources
- Mast cells, T cells, bone marrow stromal cells
- Cell Targets
- B cells, T cells, monocytes, endothelial cells, fibroblasts
- Heterodimer IL-4Rα (CD124); γ-subunit (CD132) in common with IL-2R, IL-7R, IL-13R, IL-15R
- Cell Type
- Biology Area
- Cell Biology, Stem Cells
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Boulay J, et al. 1992. Curr. Opin. Immunol. 4:294.
3. Dullens H, et al. 1991. In vivo 5:567.
4. Paul W. 1991. Blood 77:1859.
- Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
- Gene ID
- 16189 View all products for this Gene ID
- View information about IL-4 on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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