Biotin anti-mouse IL-10 Antibody anti-IL-10

Pricing & Availability

Clone: JES5-16E3 (See other available formats)
Regulatory Status: RUO
Other Names: Interleukin-10, Cytokine synthesis inhibitory factor (CSIF), B cell derived T cell growth factor (B-TCGF)
Isotype: Rat IgG2b, κ
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Product Citations: publications

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505003

50 µg

81€

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505004

500 µg

220€

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Description

IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. IL-10 inhibits IFN-γ, TNF-β, and IL-2 production by Th1 clones; inhibits macrophage-mediated IL-1, IL-6, and TNF-α synthesis; suppresses the delayed type hypersensitivity response; stimulates Th2 cell response (which results in elevated antibody production); and promotes mast cell proliferation in combination with IL-4.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

E. coli-expressed, recombinant mouse IL-10

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

ELISA Detection, ELISPOT Detection, ICFC

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA or ELISPOT detection, the antibody should be titrated between 0.25-1.0 µg/ml to determine optimal conditions. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Detection^1,9,11: The biotinylated JES5-16E3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified JES5-2A5 antibody (Cat. Nos. 504902 & 504904) as the capture antibody.
ELISA Capture: The purified JES5-16E3 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated JES5-2A5 antibody (Cat. No. 505003) as the detection antibody and recombinant mouse IL-10 (Cat. No. 575809) as the standard.
Neutralization¹⁴: The Ultra-LEAF™ purified JES5-16E3 antibody can neutralize the bioactivity of natural or recombinant IL-10.
Flow Cytometry³: The fluorochrome-labeled JES5-16E3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10-producing cells within mixed cell populations.
Additional reported applications (for relevant formats) include: immunohistochemistry³.

Application References (PubMed link indicates BioLegend citation)

Simkin G, et al. 2000. J. Immunol. 164:2457.
Kitagaki K, et al. 2002. Clin. Diagn. Lab Immunol. 9:1260.
Khanna A, et al. 2000. J. Immunol. 164:1346.
Sander B, et al. 1993. J. Immunol. Methods 166:201.
Litton M, et al. 1994. J. Immunol. Methods 175:47.
Andersson U, et al. 1999. Detection and qunatification of gene expression. New York:Springer-Verlag.
Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons New York. Unit 6.28.
Wang W, et al. 2004. FASEB J. 18:1043.
Brummel R and Lenert P. 2005. J. Immunol. 174:2429.
Lawson BR, et al. 2007. J. Immunol. 178:5366.
Xu G, et al. 2007. J. Immunol. 179:5358. PubMed
Brummel R, et al. 2005. J. Immunol.174:2429. PubMed
Kang YJ, et al. 2007. Stem Cells 25:1814. PubMed
Seo N, et al. 2001. Immunology. 103:449. (Neut)

Product Citations

Godebu E, et al. 2008. J Immunol. 181:1798. PubMed
Cox N, et al. 2015. Proc Natl Acad Sci U S A. 112: 8385 - 8390. PubMed
Campos–Mora M, et al. 2019. Front Immunol. 1.029166667. PubMed
Yan J, et al. 2020. Cell Rep. 107820:31. PubMed

RRID

AB_315357 (BioLegend Cat. No. 505003)
AB_315358 (BioLegend Cat. No. 505004)

Antigen Details

Structure: Acid-labile cytokine, dimer, 17-21 kD (Mammalian)
Cell Sources: Activated CD8⁺ T cells, Th0, Th2 subset of CD4⁺ T cells, Ly-1⁺ B cells, monocytes, macrophages, keratinocytes
Cell Targets: T cells, B cells, mast cells, macrophages
Receptors: IL-10R (CDw210)
Cell Type: Tregs
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. de Waal-Malefy R, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard M, et al. 1992. Immunol. Today 13:198.
4. Quesniaux V. 1992. Res. Immunol. 143:385.
5. Norton SK, et al. 2008. J. Immunol. 180:2848.
Regulation: Downregulated by IL-4, IL-10
Gene ID: 16153 View all products for this Gene ID
UniProt: View information about IL-10 on UniProt.org

Documentation

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Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

How many biotin molecules are per antibody structure?: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All IL-10 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-mouse IL-10	JES5-16E3	ICFC
Biotin anti-mouse IL-10	JES5-16E3	ELISA Detection,ELISPOT Detection,ICFC
FITC anti-mouse IL-10	JES5-16E3	ICFC
PE anti-mouse IL-10	JES5-16E3	ICFC
Purified anti-mouse IL-10	JES5-16E3	ELISA Capture,CyTOF®,IHC
Alexa Fluor® 647 anti-mouse IL-10	JES5-16E3	ICFC
PE/Cyanine7 anti-mouse IL-10	JES5-16E3	ICFC
Alexa Fluor® 488 anti-mouse IL-10	JES5-16E3	ICFC
Brilliant Violet 421™ anti-mouse IL-10	JES5-16E3	ICFC
Pacific Blue™ anti-mouse IL-10	JES5-16E3	ICFC
PerCP/Cyanine5.5 anti-mouse IL-10	JES5-16E3	ICFC
Purified anti-mouse IL-10 (Maxpar® Ready)	JES5-16E3	ELISA Capture,CyTOF®
Brilliant Violet 605™ anti-mouse IL-10	JES5-16E3	ICFC
PE/Dazzle™ 594 anti-mouse IL-10	JES5-16E3	ICFC
APC/Cyanine7 anti-mouse IL-10	JES5-16E3	ICFC
Ultra-LEAF™ Purified anti-mouse IL-10	JES5-16E3	ELISA Capture,CyTOF®,ELISPOT Capture,Neut,ICFC,IHC

Concentration & Expiration Lookup

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11.30.2012

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