- 11-26c.2a (See other available formats)
- Regulatory Status
- Other Names
- Immunoglobulin D
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
|Cat #||Size||Price||Quantity Check Availability||Save|
Surface IgD is an important B cell differentiation marker.Product Details
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3.
(PubMed link indicates BioLegend citation)
- Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
- Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
- Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
- Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
- Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
- Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
- Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
- Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
- Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed
- Product Citations
AB_2563343 (BioLegend Cat. No. 405733)
AB_2563344 (BioLegend Cat. No. 405734)
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.