- 30-H12 (See other available formats)
- Regulatory Status
- Other Names
- Rat IgG2b, κ
- Ave. Rating
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- Product Citations
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CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30-H12 antibody has been reported to induce Ca2+ flux in thymocytes and, in combination with antibody against the CD3/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes.Product Details
- Antibody Type
- Host Species
- Mouse thymus or spleen
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: in vivo and in vitro depletion1,2,7, costimulation of CD3/TCR-mediated signal transduction3,4, and immunohistochemical staining5 of acetone-fixed frozen sections. The 30-H12 antibody does not react with Thy-1.1 alloantigen of the AKR/J and PL strains. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301 & 101302) is recommended prior to immunofluorescent staining. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 105351 & 105352).
(PubMed link indicates BioLegend citation)
- Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
- Seaman WE. 1983. J. Immunol. 130:1713. (Deplete)
- Nakashima I, et al. 1991. J. Immunol. 147:1153. (Costim)
- Nakashima I, et al. 1993. J. Immunol. 151:3511. (Costim)
- Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (IHC)
- Hardy B, et al. 2005. Int. Immunol. 17:615.
- Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
- Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed
- Sungur CM, et al. 2013. PNAS. 110:7401. PubMed
- Product Citations
AB_313175 (BioLegend Cat. No. 105304)
- Ig superfamily, 25-35 kD
Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice
- Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells
- Cell Type
- Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
- Biology Area
- Molecular Family
- CD Molecules
- Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Craig W, et al. 1993. J. Exp. Med. 177:1331.
3. Reif AE and Schlesinger M. 1989. Cell Surface Antigen Thy-1.
4. Mayani H, et al. 1994. Blood 83:2410.
- Gene ID
- 21838 View all products for this Gene ID
- View information about CD90.2 on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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