Biotin anti-mouse CCL8 (MCP-2) Antibody

Pricing & Availability
Poly5352 (See other available formats)
Regulatory Status
Other Names
Monocyte chemotactic protein 2, MCP-2, small inducible cytokine subfamily A, member 8 (SCYA8),Chemokine (C-C motif) ligand 8, CCL8, MCP2
Goat Polyclonal IgG
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535203 50 µg 300€
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CCL8, also known as MCP-2, is a CC chemokine of the monocyte chemoattractant protein (MCP) family that plays key roles in allergic and inflammatory responses. CCL8 (MCP-2) has structural and functional similarity to other family members MCP-1 and MCP-3, and is often co-expressed with these chemokines in mononuclear cells. CCL8 is also constitutively expressed in the skin and lymph nodes of mice.

Mouse CCL8 induces a chemotactic migration of a population of differentiated Th2 cells enriched in IL-5 and IL-25 receptors by binding to the CCR8 receptor. Recent studies have established CCL8 as a critical ligand for driving CCR8-dependent Th2 cell trafficking into the skin, which in conjunction with locally recruited eosinophils drives chronic cutaneous allergic inflammation. CCL8 expression by suprabasal buldge cells has also been experimentally shown to negatively regulate the population of mouse hair follicles by Langerhans cells, further underscoring this chemokines central contributions to maintaining cutaneous immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Recombinant protein
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Detection applications, a concentration range of 0.001 - 1 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of CCL8 (MCP-2) recombinant protein ranging from 15.6 to 1,000 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The Biotin Poly5352 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with purified A16070K antibody (Cat. No. 536902) as the capture antibody and Recombinant Mouse CCL8 (MCP-2) (ELISA Std.) (Cat. No. 581709)

AB_2783340 (BioLegend Cat. No. 535203)

Antigen Details


Fibroblast, endothelial cells, monocytes, macrophages, and skeletal muscle cells.

CCL8 chemoattracts monocytes, T cells, NK cells, mast cells, eosinophils, and basophils. CCL8 is induced by IL-1β, IFNβ, IFNγ, dsRNA, virus, ConA, and anti-CD2. The induction of CCL8 by IFNγ synergizes with the TLR ligands peptidoglycan (TLR2), dsRNA (TLR3), and LPS (TLR4).
Monocytes, T cells, NK cells, mast cells, eosinophils, and basophils.
CCR1 (CD191), CCR2 (CD192), CCR3 (CD193), and CCR5 (CD195)
Cell Type
Endothelial cells, Fibroblasts, Macrophages, Monocytes
Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Antigen References
  1. Van Damme J, et al. 1992. J. Exp. Med. 176:59.
  2. Proost P, et al. 1998. J. Immunol. 160:4034.
  3. Dean RA, et al. 2008. Blood 112: 3455.
  4. Hori T, et al. 2008. Blood 111:4403.
  5. Rom S, et al. 2010. FASEB J. 24:2292.
  6. Struyf S, et al. 2009. Eur. J. Immunol. 39:843.
  7. Islam SA, et al. 2011. Nat. Immunol. 12:167.
Gene ID
20307 View all products for this Gene ID
View information about CCL8 on

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 12.11.2018

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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  • Biotin anti-mouse CCL8 (MCP-2)


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