ICC staining of Biotin anti-MFF antibody (clone N328/14) on HeLa cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum and 0.02% bovine serum albumin. The cells were then stained with 5.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 Streptavidin (Cat. No. 405240) for 1 hour at room temperature. The cells were counterstained with Flash Phalloidin™ Green 488 (Cat. No. 424201) and DAPI to visualize actin filaments and nuclei, respectively. The image was captured with a 60X objective. Scale bar: 20 µm
ICC staining of Biotin anti-MFF antibody (clone N328/14) on HeLa cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum and 0.02% bovine serum albumin. The cells were then stained with 5.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 Streptavidin (Cat. No. 405240) for 1 hour at room temperature. The cells were counterstained with Flash Phalloidin™ Green 488 (Cat. No. 424201) and DAPI to visualize actin filaments and nuclei, respectively. The image was captured with a 60X objective. Scale bar: 20 µm
ICC staining of Biotin anti-MFF antibody (clone N328/14) on A549 cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum and 0.02% bovine serum albumin. The cells were then stained with 5.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 Streptavidin (Cat. No. 405240) for 1 hour at room temperature. The cells were counterstained with Flash Phalloidin™ Green 488 (Cat. No. 424201) and DAPI to visualize actin filaments and nuclei, respectively. The image was captured with a 60X objective. Scale bar: 20 µm.
Mitochondrial fission factor (MFF) is a mitochondrion outer membrane protein that is part of the mitochondrial fission complex where it acts a receptor for the dynamin-related protein 1 (Drp1). The MFF-Drp1 complex is responsible for the promotion of mitochondrial fission. MFF has also been shown to regulate peroxisome morphology.
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
MFF is a 342 amino acid protein with a molecular mass of ~38 kD.
Distribution
Tissue Distribution: Highly expressed in heart, kidney, liver, brain, muscle, and stomach.
Cellular Distribution: Mitochondria, peroxisome, nucleus, and cytosol.
Function
MFF plays a role in mitochondrial and peroxisomal fission by facilitating the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface.
Interaction
MFF interacts with the dynamin-related protein 1 (Drp1). MFF has also been shown to interact with DDX28 and ILF3.
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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