Biotin anti-c-Myc Antibody (Previously Covance catalog# BIOT-150L)

Pricing & Availability
9E10 (See other available formats)
Regulatory Status
Other Names
myc tag, c-Myc tag, cMyc tag, Myc epitope tag
Covance Catalog# BIOT-150L
Mouse IgG1, κ
Ave. Rating
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Product Citations
Western blot analysis of 1, 0.5, 0.1 µl (lanes 1, 2, 3 respectively) of Posi-tag (Cat. No. 931301) using Biotin anti C-Myc Mouse primary antibody (Clone 9E10, 1:2500 dilution, 0.4 µg/ml) and HRP Avidin secondary antibody (Cat. No. 405103, 1:1000 dilution).
  • 9E10_c-myc_epitope_tag_1_011918
    Western blot analysis of 1, 0.5, 0.1 µl (lanes 1, 2, 3 respectively) of Posi-tag (Cat. No. 931301) using Biotin anti C-Myc Mouse primary antibody (Clone 9E10, 1:2500 dilution, 0.4 µg/ml) and HRP Avidin secondary antibody (Cat. No. 405103, 1:1000 dilution).
  • 9E10_c-myc_epitope_tag_2_011918
    Immunofluorescence of Myc-tagged plasmid-transfected A549 cells with (A) mouse IgG1, κ isotype control (Negative, Cat. No. 401402) or (B-D) Biotin-Myc Mouse primary antibody (Clone 9E10). Alexa Fluor® 594 (Red) Streptavidin (Cat. No. 405240) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A) is 1/30, (B, D) is 1/600 and (C) is 1/900. Concentrations for (A-C) is 1 µg/ml, (D) is 0.5 µg/ml.
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Cat # Size Price Quantity Check Availability Save
908805 100 µL 336€
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The 9E10 monoclonal antibody recognizes the amino acid sequence EQKLISEEDL, which is a specific portion of the human c-myc gene product. This antibody is highly specific and is unlikely to alter the activity of the cloned sequence.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
All Species
Antibody Type
Host Species
Amino acids 408-439, C-terminal region of human c-myc
Phosphate-buffered solution + 0.03% Thimerosal + 50% Glycerol.
The antibody was purified by affinity chromatography.
1 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

WB - Quality tested
ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.4 µg per ml (1:2500 dilution). For immunocytochemistry, a concentration range of 0.5 - 1.0 μg/ml (1:1000-1:2000) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Rao H, et al. 2001. Nature. 410:955.
  2. Peters CS, et al. 2001. J Biol Chem. 276:13718.
  3. Munro S, Pelham HR. 1987. Cell. 48:899.
  4. Evan GI, et al. 1985. Mol Cell Biol. 5:3610.
Product Citations
  1. Rad S M AH, et al. 2020. PLoS One. 15:e0232915. PubMed
  2. Marshall RS, et al. 2020. J Cell Sci. 133:jcs249862. PubMed
  3. Parodi E, et al. 2015. PLoS One. 10: 0143571. PubMed
  4. Marshall RS, et al. 2020. J Cell Sci. :133. PubMed
AB_2565090 (BioLegend Cat. No. 908805)

Antigen Details

Biology Area
Cell Biology, Protein Purification, Protein Synthesis
Antigen References
  1. Adams JM, et al. 1983. Proc. Natl. Acad. Sci. USA 80:1982.
  2. Atchley WR, et al. 1995. Proc. Natl. Acad. Sci. USA 92:10217.
  3. Battey J, et al. 1983. Cell 34:779.
  4. Beimling P, et al. 1985. Biochemistry 24:6349.
Gene ID
4609 View all products for this Gene ID
View information about c-Myc on

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 9    Revision Date: 07.03.2023

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-c-Myc

  • Anti-c-Myc Tag (9E10) Affinity Gel

  • Biotin anti-c-Myc

  • Direct-Blot™ HRP anti-c-Myc

  • Alexa Fluor® 594 anti-c-Myc

  • Ultra-LEAF™ Purified anti-c-Myc

  • Alexa Fluor® 647 anti-c-Myc

  • Alexa Fluor® 488 anti-c-Myc Antibody


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