Biotin anti-α-Synuclein, 103-108 Antibody

Pricing & Availability
Clone
4B12/Synuclein (See other available formats)
Other Names
NACP, PARK1, PARK4, PD1, Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-A beta component of AD amyloid Parkinson disease
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
4B12slashSynuclein_Purified_alpha_Synuclein_103-108_1_120717
IHC staining of Biotin anti-α-Synuclein, 103-108 antibody (clone 4B12/Synuclein) on formalin-fixed paraffin-embedded normal (A) and Parkinson’s disease (B) brain tissue. Following antigen retrieval using 88% formic acid, tissues were incubated with 1 µg/mL of the conjugated antibody overnight at 4°C. For detection, the HRP labeling reagent and DAB from BioLegend Ultra Streptavidin (USA) HRP Detection Kit was used (Multi-Species, component #5, DAB; Cat. No. 929901). The slides were counterstained with hematoxylin and bluing solution. The images were captured using a 40X objective.
  • 4B12slashSynuclein_Purified_alpha_Synuclein_103-108_1_120717
    IHC staining of Biotin anti-α-Synuclein, 103-108 antibody (clone 4B12/Synuclein) on formalin-fixed paraffin-embedded normal (A) and Parkinson’s disease (B) brain tissue. Following antigen retrieval using 88% formic acid, tissues were incubated with 1 µg/mL of the conjugated antibody overnight at 4°C. For detection, the HRP labeling reagent and DAB from BioLegend Ultra Streptavidin (USA) HRP Detection Kit was used (Multi-Species, component #5, DAB; Cat. No. 929901). The slides were counterstained with hematoxylin and bluing solution. The images were captured using a 40X objective.
  • 4B12slashSynuclein_Purified_alpha_Synuclein_103-108_2_120717
    Western blot of Biotin anti-α-Synuclein, 103-108 antibody (clone 4B12/Synuclein). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 10 ng of recombinant human α-Synuclein protein. The blot was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Streptavidin (Cat. No. 405210) for 60 minutes at room temperature. Enhanced chemiluminescence was used as the detection system.
  • 4B12slashSynuclein_Purified_alpha_Synuclein_103-108_3_120717
Cat # Size Price Quantity Avail. Save
807807 25 µg 84€
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807808 100 µg 208€
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Description

α-Synuclein is expressed principally in the nervous system, but it is also produced in other tissues, including  the skin. In the brain, the protein is primarily neuronal, but it is also present in glia. Neuronal α-synuclein is concentrated in presynaptic nerve terminals, interacts with plasma membrane phospholipids, and is also present in nuclei and mitochondria. At least three isoforms of synuclein are produced through alternative splicing. The most common isoform is a 140 amino acid-long transcript. Other isoforms are a-synuclein-126, lacking residues 41-54; and α-synuclein-112, which lacks residues 103-130. α-Synuclein’s physiological role is poorly understood, but the protein has been implicated in regulating dopamine release and transport, synaptic vesicle clustering, and functioning as a SNARE-complex chaperone. α-Synuclein fibrils are major component of the intracellular Lewy bodies that are associated with Parkinson's disease, Lewy body dementia, and multiple system atrophy.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was developed using a purified, E. coli produced human α-synuclein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

IHC-P - Quality tested
WB, ELISA Detection - Validated

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For ELISA Detection applications, a concentration range of 0.5 -2.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of α-synuclein recombinant protein ranging from 6 to 1500 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody reacts with human α-synuclein. It does not react with mouse or rat α-synuclein. The epitope lies within amino acids 103-108 of α-synuclein.

Additional reported applications (for the relevant formats) include: ELISA2, Western blotting1,2,3,4,6,8, and immunoprecipitation4.

Application References

(PubMed link indicates BioLegend citation)
  1. Lahut S, et al. 2017. Dis Model Mech(WB) PubMed
  2. Landeck N, et al. 2016. Mol Neurodegener. 11(1): 61. (ELISA, WB) PubMed
  3. Moussaud S, et al. 2015. Expert Opin Ther Targets. 19(5): 589. (WB) PubMed
  4. Helferich AM., et al. 2015. Mol Neurodegener. 10(1): 66. (IHC, IP, WB) PubMed
  5. Bodis‐Wollner I, et al. 2014. Ann Neurol. 75(6): 964. (IHC)
  6. Shen W, et al. 2014. J Neurochem. 129(5): 884. (WB)
  7. Fagerqvist T, et al. 2013. J Neurochem. 126(1): 131. (IHC)
  8. Ebrahimi-Fakhari D, et al. 2011. J Neurosci. 31(41): 14508. (WB) PubMed
  9. Welander H, et al. 2011. Biochem Biophys Res Commun. 412(1): 32. (IHC)
RRID
AB_2721318 (BioLegend Cat. No. 807807)
AB_2721319 (BioLegend Cat. No. 807808)

Antigen Details

Structure
α-Synuclein’s canonical isoform is a 140 amino acid protein with an apparent molecular mass of 14 kD.
Distribution

Tissue distribution: Primarily nervous system, but lower expression in other tissues.
Cellular distribution: Cytoskeleton, cytosol, lysosome, mitochondria, nucleus, plasma membrane, and extracellular.

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
α-Synuclein
Antigen References
  1. Lee JM, et al. 2017. J Neuropathol Exp Neurol. 76(1):2.
  2. Kang SS, et al. 2017. Proc Natl Acad Sci. 114(5): 1183.
  3. Valdes P, et al. 2016. Front Neuroanat. 10:123.
  4. Emamzadeh FN. 2016. J Res Med Sci. 9(21):29.
Gene ID
6622 View all products for this Gene ID
UniProt
View information about α-Synuclein, 103-108 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 12.14.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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