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Avidin/Biotin Blocking System (Previously Covance catalog# SIG-31126)

Pricing & Availability
Regulatory Status
RUO
Other Names
Avidin/Biotin Blocking Kit
Previously
Covance Catalog# SIG-31126
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
927301 2 x 15 ml 83€
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Technical Data Sheet (pdf)

Kit Contents

Kit Contents

• Avidin Solution, 15 mL
• Biotin Solution, 15 mL

Both reagents are provided ready-to-use and need no dilution

Product Details

Formulation
Blocking Kit. The Avidin and Biotin Solutions contain 0.1% sodium azide.
Storage & Handling
Store between 2°C and 8°C.
Application

IHC, WB
Recommended Usage

The optimal working dilution should be determined for each specific assay condition.

Blocking Procedure: 
For paraffin-embedded tissues: To be performed after the antigen retrieval and serum block but before primary antibody incubation.

1. Incubate the Avidin Solution for 10-15 minutes at room temperature.
2. Incubate with the Biotin Solution for 10-15 minutes at room temperature.

Application Notes

BioLegend's Avidin/Biotin Blocking System is intended for use in immunohistochemistry and immunoblotting procedures. The purpose is to decrease the signal caused by endogenous avidin, biotin, or biotin-binding proteins when immunostaining specimens with detection systems that rely on avidin-biotin or steptavidin-biotin chemistry. Nonspecific staining due to these factors commonly resembles a diffuse, weak, cytoplasmic pattern.

Product Citations
  1. Mooney JP, et al. 2022. Wellcome Open Res. 7:134. PubMed
  2. Klarquist J, et al. 2021. Cell Rep. 36:109591. PubMed
  3. Ferrian S, et al. 2021. Cell Rep Med. 2:100419. PubMed
  4. Risom T, et al. 2022. Cell. 185:299. PubMed
  5. Scur M, et al. 2022. Nat Commun. 13:7272. PubMed
  6. Canedo EC, et al. 2021. JCI Insight. 6:. PubMed
  7. Elhanani O, et al. 2021. Methods Mol Biol. 2386:147. PubMed

Antigen Details

Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 03.29.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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