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APC anti-mouse Notch 1 Antibody

Pricing & Availability
Clone
HMN1-12 (See other available formats)
Regulatory Status
RUO
Other Names
Neurogenic locus notch homolog protein 1
Isotype
Armenian Hamster IgG
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Product Citations
publications
HMN1-12_APC_1_021111
C57BL/6 mouse CD4 and CD8 double-negative thymocytes were stained with CD24 PE and Notch1 (HMN1-12) APC (top) or Armenian hamster IgG APC isotype control (bottom).
  • HMN1-12_APC_1_021111
    C57BL/6 mouse CD4 and CD8 double-negative thymocytes were stained with CD24 PE and Notch1 (HMN1-12) APC (top) or Armenian hamster IgG APC isotype control (bottom).
  • HMN1-12_APC_2_021111
Compare all formats See APC spectral data
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130613 25 µg 129€
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Description

The Notch receptors are highly conserved from invertebrates to mammals. Notch 1 and Notch 2 exhibit the greatest structural similarity among the four mammalian Notch receptors. Notch 3 has a number of structural and functional differences. The binding of Notch 3 to its ligands results in the proteolysis of Notch and movement of intracellular portions of Notch into the nucleus. This translocation triggers a series of signaling processes. Notch 3 is primarily expressed in adult arterial smooth muscle cells. Notch 3 gene mutation can cause CADASIL, an inherited early stroke syndrome.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Notch1-Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Moriyama Y, et al. 2008. Int. Immunol. 20:763.
Product Citations
  1. Burghardt S, et al. 2013. J Immunol. 191:5574. PubMed
  2. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
  3. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  4. Yu S, et al. 2018. Cell Stem Cell. 23:46. PubMed
RRID
AB_10897096 (BioLegend Cat. No. 130613)

Antigen Details

Structure
Transmembrane receptor. Heterodimer consisting of a C-terminal fragment and N-terminal fragment probably linked by disulphide bonds. Contains 5 ankyrin repeats, 29-36 EGF repeats, 3 Notch/Lin repeats.
Distribution

Highly expressed in the brain, lung, and thymus (CD4-CD8- cells and CD4-CD8+ cells). Lower levels of expression in spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney, and heart.

Function
Regulates broad spectrum of various cell lineage commitment processes, including myogenesis, neurogenesis, gliogenesis, and lymphocyte development.
Ligand/Receptor
Jag 1,2; Delta 1,2
Cell Type
Neural Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology
Molecular Family
Postsynaptic proteins
Antigen References

1. Ehebauer ME, et al. 2006. Biochem. J. 392:13.
2. Shimizu K, et al. 2000. Mol. Cell. Biol. 20:18.
3. Tanigaki K, et al. 2007. Nature Immunol. 8:4514.
4. Kraman M, et al. 2005. FASEB J. 19:1311.

Gene ID
18128 View all products for this Gene ID
UniProt
View information about Notch 1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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