APC anti-mouse CD86 Antibody

Pricing & Availability
Clone
GL-1 (See other available formats)
Regulatory Status
RUO
Other Names
B7-2, B70, Ly-58
Isotype
Rat IgG2a, κ
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Product Citations
publications
GL-1_APC_090707
LPS-stimulated (3 days) C57BL/6 mouse splenocytes stained with GL-1 APC
  • GL-1_APC_090707
    LPS-stimulated (3 days) C57BL/6 mouse splenocytes stained with GL-1 APC
See APC spectral data
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105011 25 µg 64,00€
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105012 100 µg 192,00€
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Description

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
LPS-activated CBA/Ca mouse splenic B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The GL-1 antibody can block the mixed lymphocyte reaction in vitro and has been shown to inhibit the priming of cytotoxic T lymphocytes in vivo (along with antibodies against B7-1). Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections2,6, immunofluorescence microscopy, and in vivo and in vitro blocking of T cell responses1-6. GL-1 is not suitable for immunohistochemical staining of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105051-105056).

Application References

(PubMed link indicates BioLegend citation)
  1. Hathcock KS, et al. 1993. Science 262:905. (Block, IP)
  2. Inaba KM, et al. 1994. J. Exp. Med. 180:1849. (Block, IHC)
  3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
  4. Krummel MF, et al. 1995. J. Exp. Med. 182:459. (Block)
  5. Liu Y, et al. 1997. J. Exp. Med. 185:251. (Block)
  6. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  9. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
  10. Klinger MB, et al. 2007. Am. J. Physiol. Requl. Integr. Comp. Physiol. 293:R677. PubMed
  11. de Verteuil DA, et al. 2014. J Immunol. 193:1121. PubMed
Product Citations
  1. Bhattacharjee P, et al. 2018. Sci Rep. 13:e0199785. PubMed
  2. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  3. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  4. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  5. Zhao F et al. 2018. Immunity. 48(1):147-160 . PubMed
  6. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  7. Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
  8. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  9. Liu WL, et al. 2019. Nat Commun. 10:3199. PubMed
  10. Arora H, et al. 2019. Immunity. 50:418. PubMed
  11. Finkin S et al. 2019. Immunity. 51(2):324-336 . PubMed
  12. Xiao Y, et al. 2019. JCI Insight. 4:e99052. PubMed
  13. Kashyap AS, et al. 2019. Cell Rep. 28:3367. PubMed
  14. Qi S, et al. 2020. Theranostics. 10:1814. PubMed
  15. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  16. Yang C, et al. 2019. Nanomedicine (Lond). 14:2423. PubMed
  17. He J, et al. 2020. Evid Based Complement Alternat Med. 2020:5637507. PubMed
  18. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  19. Yamada KJ, et al. 2020. PLoS Pathog. 16:e1008354. PubMed
  20. Mul Fedele ML, et al. 2020. Front Cell Infect Microbiol. 10:100. PubMed
  21. Yu X, et al. 2020. Cancer Cell. 37(6):850-866. PubMed
  22. Lu SW, et al. 2020. Nat Commun. 3.660416667. PubMed
  23. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  24. Coronel MM, et al. 2020. Sci Adv. 6:eaba5573. PubMed
  25. Shepherd J 2011. Toxicol Sci. 120:68. PubMed
  26. Verteuil D, et al. 2014. J Immunol. 193:1121. PubMed
  27. Seki T, et al. 2015. J Biochem. 158: 485 - 495. PubMed
  28. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  29. Sellau J, et al. 2016. Sci Rep. 6:28058. PubMed
  30. Gong N, et al. 2020. Nat Nanotechnol. 1.35625. PubMed
  31. Trivedi S, et al. 2020. Elife. 9:00. PubMed
  32. Ukidve A, et al. 2020. Proc Natl Acad Sci U S A. 117:17727. PubMed
  33. Bi CS, et al. 2020. Cell Prolif. 53:e12827. PubMed
  34. Huang L, et al. 2021. Frontiers in Cellular and Infection Microbiology. 10:606340. PubMed
  35. Dai X, et al. 2021. Molecular Cell. 81(11):2317-2331.e6. PubMed
  36. Gozgit JM, et al. 2021. Cancer Cell. :. PubMed
  37. Oda SK, et al. 2020. J Exp Med. 217: . PubMed
  38. Han F, et al. 2020. Invest Ophthalmol Vis Sci. 61:24. PubMed
  39. Zheng B, et al. 2021. Bioact Mater. 6:3879. PubMed
  40. Yang Z, et al. 2021. Nat Commun. 12:4299. PubMed
RRID
AB_493343 (BioLegend Cat. No. 105011)
AB_493342 (BioLegend Cat. No. 105012)

Antigen Details

Structure
Ig superfamily, 80 kD
Distribution

B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes

Function
T cell costimulation, Ig class-switching, NK cell cytotoxicity
Ligand/Receptor
CD28, CD152 (CTLA-4)
Cell Type
Astrocytes, B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Hathcock KS, et al. 1993. Science 262:905.
3. Freeman GJ, et al. 1993. Science 262:907.
4. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.

Gene ID
12524 View all products for this Gene ID
UniProt
View information about CD86 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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