APC anti-mouse CD200 (OX2) Antibody

Pricing & Availability
Clone
OX-90 (See other available formats)
Other Names
OX-2, MRC
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
OX-90_APC_1_062911
C57BL/6 mouse splenocytes were stained with CD45R/B220 PE and CD200 (clone OX-90) APC (top), or rat IgG2a, κ APC isotype control (bottom).
  • OX-90_APC_1_062911
    C57BL/6 mouse splenocytes were stained with CD45R/B220 PE and CD200 (clone OX-90) APC (top), or rat IgG2a, κ APC isotype control (bottom).
  • OX-90_APC_2_062911
See APC spectral data
Cat # Size Price Quantity Avail. Save
123809 25 µg 72€
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123810 100 µg 172€
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Description

CD200 (OX-2 antigen) is a type-1 membrane glycoprotein containing two extracellular Ig-like domains. CD200 a highly conserved type I membrane glycoprotein that is expressed on a variety of cell types including thymocytes, some T cells, endothelial and follicular dendritc cells, B cells, and brain tissue (neurons); but not on NK cells, granulocytes, monocytes, or macrophages. CD200 costimulates T cell proliferation. It may regulate myeloid cell activity in a variety of tissues. CD200 is the ligand for CD200 receptor (CD200R). The CD200 Receptor is restricted to myeloid cells, and it is believed that its engagement with CD200 results in inhibition and/or downregulation of myeloid cell activity. Blocking of CD200/CD200R interactions decreases myeloid cell inhibitory thresholds which results in enhanced immune activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Soluble fusion protein of the extracellular region of mouse OX-2 antigen with domains 3 and 4 of rat CD4 fusion protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed
  2. Wakabayashi T et al. 2018. Cell stem cell. 22(3):384-397 . PubMed
  3. Han Y, et al. 2019. J Clin Invest. 130:26. PubMed
  4. Wang L, et al. 2020. Nat Commun. 0.654166667. PubMed
  5. Kim JM, et al. 2020. Nat Commun. 11:2289. PubMed
  6. Kathiriya JJ, et al. 2020. Cell Stem Cell. 26(3):346-358.e4.. PubMed
  7. Najar M, et al. 2012. Immunol Lett. 146:50. PubMed
  8. Feng H, et al. 2020. J Clin Invest. 130:6354. PubMed
  9. Grol MW, et al. 2021. eLife. 10:00. PubMed
RRID
AB_10900996 (BioLegend Cat. No. 123809)
AB_10900447 (BioLegend Cat. No. 123810)

Antigen Details

Structure
Immunoglobulin (Ig) superfamily
Distribution

Thymocytes, some T cells, endothelial, B cells

Function
Co-stimulatory factor in T cell proliferation
Ligand/Receptor
CD200R
Antigen References

1. Hoek RM, et al. 2000. Science 290:1768.
2. Gorczynski R, et al. 2004. J. of Immunol. 172:7744.
3. Gorczynski L, et al. 1999. J. Immunol. 162:774.
4. Rosenblum MD, et al. 2004. Blood 103:2691.
5. Zhang S, et al. 2004. J. of Immunol. 173:6786.
6. Barclay AN, et al. 2002. Trends Immunol. 23:285.

Gene ID
4345 View all products for this Gene ID
UniProt
View information about CD200 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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