APC anti-human/mouse CD49f Antibody

Pricing & Availability
Clone
GoH3 (See other available formats)
Workshop
HCDM listed
Other Names
VLA-6 α chain, α6 integrin, integrin α6, ITGA6
Isotype
Rat IgG2a, κ
Ave. Rating
1 reviews
Product Citations
publications
GOH3_APC_102110
Human peripheral blood lymphocytes were stained with anti-human/mouse CD49f (clone GOH3) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
  • GOH3_APC_102110
    Human peripheral blood lymphocytes were stained with anti-human/mouse CD49f (clone GOH3) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
See APC spectral data
Cat # Size Price Quantity Avail. Save
313615 25 tests 100€
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313616 100 tests 212€
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Description

CD49f is a 120 kD integrin family member also known as VLA-6 α chain and α6 integrin subunit. CD49f associates with either integrin β1 (CD29) or integrin β4 (CD104) to form receptors (VLA-6 or α6β4 complex) for laminin and kalinin. CD49f is expressed on platelets, monocytes, T cells, placental trophoblasts, and epithelial and endothelial cells. CD49f is involved in adhesion and can act as a co-stimulatory molecule for T cell activation and proliferation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Mouse, Baboon, Capuchin Monkey, Cat (Feline), Cattle (Bovine, Cow), Chimpanzee, Cynomolgus, Dog (Canine), Horse (Equine), Rabbit (Lapine), Rhesus, Sheep (Ovine), Swine (Pig, Porcine)
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse mammary tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,5, in vitro and in vivo blocking of cell binding to laminin and blocking the function of integrin α61,4, and immunohistochemistry of acetone-fixed frozen sections2,3,5. The GoH3 antibody has been reported to block laminin binding in vitro and to block integrin α6 function in vivo.

Application References

(PubMed link indicates BioLegend citation)
  1. Georas SN, et al. 1993. Blood 82:2872. (IP, Block)
  2. Honda T, et al. 1995. J. Clin. Endocrinol. Metab. 80:2899. (IHC)
  3. Sonnenberg A, et al. 1986. J. Histochem. Cytochem. 34:1037. (IHC)
  4. Nakamura K, et al. 1997 Biochem. Biophys. Res. Commun. 235:524. (Block)
  5. Sonnenberg A, et al. 1987 J. Biol. Chem. 262:10376. (IP, IHC)
  6. Deregibus MC, et al. 2007. Blood doi:10.1182/blood-2007-03-078709.
  7. Horwitz KB, et al. 2008. Proc Natl Acad Sci USA. 105:5774. PubMed
  8. Nardella C, et al. 2009. Sci Signal. 2:55. PubMed
  9. Xu T, et al. 2010. Mol Cancer Ther. 9:438. PubMed
  10. Stepp MA, et al. 2007. J Cell Sci. 120:2851. PubMed
  11. Jo M, et al. 2010. Cancer Res. 70:8948. PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Grange C, et al. 2011. Cancer Res. 71:5346. PubMed
  14. Lai KP, et al. 2012. Mol Endocrinol. 26:52. PubMed
  15. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
Product Citations
  1. Morimoto H, et al. 2009. PLoS One. 4:e7909. PubMed
  2. Volkmer J, et al. 2012. Proc Natl Acad Sci U S A. 109:2078. PubMed
  3. Saen D, et al. 2013. Fertil Steril. 99:1264. PubMed
  4. Szade K, et al. 2013. PLoS One. 8:63329. PubMed
  5. Chang J, et al. 2013. J Biol Chem. 288:28952. PubMed
  6. Chang J, et al. 2013. Stem Cell Res. 11:990. PubMed
  7. Saenz F, et al. 2014. PLoS One. 9:97666. PubMed
  8. Li Y, et al. 2014. Cell Death Dis. 5:1550. PubMed
  9. Cichon M, et al. 2016. Cancer Res . 76: 3520 - 3530. PubMed
  10. Skowron K, et al. 2016. Sci Rep. 6:35854. PubMed
  11. Liu S, et al. 2016. PLoS One. 11:e0163232. PubMed
  12. Dall G, et al. 2017. Stem Cell Reports. 8(2):417-431. PubMed
  13. Han Y, et al. 2019. J Clin Invest. 130:26. PubMed
  14. Cai S et al. 2017. Cell stem cell. 20(2):247-260 . PubMed
  15. Haas TL et al. 2017. Cell stem cell. 21(1):35-50 . PubMed
RRID
AB_2734290 (BioLegend Cat. No. 313615)
AB_1575047 (BioLegend Cat. No. 313616)

Antigen Details

Structure
Integrin family, associates with β1 or β4, 120 kD
Distribution

Platelets, monocytes, T cells, placental trophoblasts, epithelial and endothelial cells

Function
Adhesion, receptor for laminin and kalinin; laminin binding to VLA-6 induces T cell co-stimulation for proliferation and activation
Ligand/Receptor
With integrin β1 (CD29) forms VLA-6, with integrin β4 (CD104) forms a6β4 integrin; laminin and kalinin are ligands for these receptors
Cell Type
Embryonic Stem Cells, Endothelial cells, Epithelial cells, Monocytes, Platelets, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Sonnenberg A, et al. 1990. J. Cell Biol. 110:2145.
2. Sonnenberg A, et al. 1990. J. Cell. Sci. 96:207.
3. Aumailley M, et al. 1990. Exp. Cell Res. 188:55.
4. Niessen CM, et al. 1994. Exp. Cell Res. 211:360.

Gene ID
16403 View all products for this Gene ID 3655 View all products for this Gene ID
UniProt
View information about CD49f on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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