APC anti-human CD271 (NGFR) Antibody

Pricing & Availability
Clone
ME20.4 (See other available formats)
Regulatory Status
RUO
Other Names
p75NTR, TNFRSF16, p75(NTR), Gp80-LNGFR, NGFR, Nerve Growth Factor Receptor
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
ME20dot4_APC_040810
Human neuroblastoma cell line, SK-N-MC, stained with ME20.4 APC
  • ME20dot4_APC_040810
    Human neuroblastoma cell line, SK-N-MC, stained with ME20.4 APC
See APC spectral data
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345107 25 tests 104,00€
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345108 100 tests 220,00€
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Description

CD271, also known as p75NTR, TNFRSF16, p75(NTR), Gp80-LNGFR, and NGFR, is a type I transmembrane protein with a MW of 75 kD. It is expressed by many cell types including neurons, Schwann cells, mesenchymal stem/stromal cells, follicular dendritic cells and melanocytes. The extracellular portion contains four TNFR-Cys repeats that form the binding domain for its ligands (NGF, BDNF, NTF3, and NTF4). The intracellular portion of CD271 contains a death domain, which interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9, to promote cell apoptosis, and to regulate cell differentiation and neurogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
WM245 melanoma cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications for the relevant formats include: immunoprecipitation1, Western blotting4, immunohistochemical staining of frozen or paraffin embedded tissue sections1,2, and blocking of NGF binding1.

Application References

(PubMed link indicates BioLegend citation)
  1. Ross A, et al. 1984. P. Natl. Acad. Sci. USA 81:6681. (FC, IP, Block, IHC)
  2. Cattoretti G, et al. 1993. Blood 81:1726. (IHC)
  3. Kamke MR, et al. 2005. Hear Res. 206:89.
  4. Baker D, et al. 1989. Cancer Res. 49:4142-4146. (FC, WB)
Product Citations
  1. Giambra V et al. 2018. Cell stem cell. 23(5):714-726 . PubMed
  2. Torre E et al. 2018. Cell systems. 6(2):171-179 . PubMed
  3. Barbarani G, et al. 2019. Sci Rep. 9:3388. PubMed
  4. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  5. Greenwood EJD et al. 2019. Cell Rep. 27(5):1579-1596 . PubMed
  6. Kumar B, et al. 2018. Cancer Res. 78:5107. PubMed
  7. Fahl SP, et al. 2018. J Immunol. 200:3450. PubMed
  8. Loo CS, et al. 2020. Immunity. 53:143. PubMed
  9. Bazot Q, et al. 2014. Nucleic Acids Res. 42:9700. PubMed
  10. Brun J, et al. 2015. PLoS One. 10: 0145153. PubMed
  11. Gu P, et al. 2016. Oncogene. 10.1038/onc.2016.405. PubMed
  12. Hu Y, et al. 2016. J Exp Med. 213: 2759 - 2772. PubMed
  13. Peng K, et al. 2020. Sci Rep. 10:18433. PubMed
  14. Boshuizen J, et al. 2020. Nat Commun. 3.198611111. PubMed
  15. Hawkins FJ, et al. 2020. Cell Stem Cell. 28(1):79-95.e8. PubMed
  16. Portillo AL, et al. 2021. iScience. 24(6):102619. PubMed
  17. Jiang M, et al. 2021. Developmental Cell. 56(11):1646-1660.e5. PubMed
RRID
AB_10639737 (BioLegend Cat. No. 345107)
AB_10645515 (BioLegend Cat. No. 345108)

Antigen Details

Structure
Type I transmembranal protein with a MW of 75 kD. The extracellular portion contains four TNFR-Cys repeats that form the NGF binding domain; the intracellular portion contains a death domain.
Distribution

Expressed by many cell types including neurons, Schwann cells, mesenchymal stem/stromal cells, follicular dendritic cells, melanocytes.

Function
Apoptosis, differentiation, neurogenesis.
Interaction
TRAF2, TRAF4, TRAF6, PTPN13, RANBP9.
Ligand/Receptor
Nerve growth factor (NGF), Brain-derived neurotrophic factor (BDNF), Neurotrophin 3. (NTF3) , Neurotrophin 4 (NTF4)
Bioactivity
Apoptosis, differentiation, neurogenesis.
Cell Type
Dendritic cells, Mesenchymal cells, Mesenchymal Stem Cells, Neurons
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, Postsynaptic proteins
Antigen References

1. Friedman MJ, et al. 2009. J. Biol. Chem. 284:27944.
2. Rock JR, et al. 2009. Proc Natl Acad Sci USA. 106:12771.
3. Kidd SK, et al. 2008. Brain Res.1195:113.
4. Peters EM, et al. 2007. Am J Pathol. 171:1872.
5. Jansen P, et al. 2007. Nat Neurosci. 10:1449.

Gene ID
4804 View all products for this Gene ID
UniProt
View information about CD271 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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