- 1A5 (See other available formats)
- Regulatory Status
- VII 70443
- Other Names
- OBBP2, CD33L2, OB-BP2
- Mouse IgG1, κ
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- Product Citations
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CD170, also known as Siglec-5, is a single pass transmembrane protein member of the immunoglobulin superfamily. On the cell surface, CD170 forms a 140 kD dimer. The cytoplasmic domain of Siglec-5 contains two ITIM motifs that recruit the tyrosine-phosphatases SHP-1 and SHP-2 after tyrosine-phosphorylation, which in turn results in the inhibition of cell signaling. Siglec-5 is expressed by granulocytes, monocytes/macrophages, subsets of lymphocytes, and a subset of activated dendritic cells. Siglec-5 binds α2,3- and α2,6-linked sialic acid as well as glycophorin A, and is involved in cell adhesion.Product Details
- Human, Cross-Reactivity: Chimpanzee, Bonobo, Gorilla2
- Antibody Type
- Host Species
- Extracellular region of Siglec-5
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
Red Laser (633 nm)
- Application Notes
The 1A5 antibody cross-reacts with Siglec-144. Additional reported applications (for the relevant formats) include: ELISA2.
(PubMed link indicates BioLegend citation)
- Cornish AL, et al. 1998. Blood 92:2123.
- Nguyen DH, et al. 2006. P. Natl. Acad. Sci. USA 103:7765. (FC, ELISA)
- Connolly NP, et al. 2002. Br. J. Haematol. 119:221.
- Angata T, et al. 2006. FASEB J. 20:1964.
- Avril T, et al. 2005. J. Biol. Chem. 280:19843.
AB_2564261 (BioLegend Cat. No. 352005)
AB_2564262 (BioLegend Cat. No. 352006)
- Member of the immunoglobulin superfamily, single pass transmembrane protein, forms a 140 kD dimer; cytoplasmic domain contains two ITIM motifs
- Granulocytes, monocytes/macrophages, subsets of lymphocytes, and activated dendritic cells
- Inhibitory receptor, cell adhesion
- SHP-1, SHP-2
- α2,3- and α2,6-linked sialic acid, glycophorin A
- Cell Type
- Granulocytes, Monocytes, Macrophages, Lymphocytes, Dendritic cells, B cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules, Siglec Molecules
- Antigen References
1. Soto PC, et al. 2010. J. Immunol. 184:4185.
2. Carlin AF, et al. 2009. J. Exp. Med. 206:1691.
3. Zhuravleva MA. 2008. J. Mol. Biol. 375:437.
- Gene ID
- 8778 View all products for this Gene ID
- View information about CD170 on UniProt.org
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