APC anti-human CD158d (KIR2DL4) Antibody

Pricing & Availability
mAb 33 (33) (See other available formats)
Regulatory Status
Other Names
KIR2DL4, 2DL4, p49, KIR103
Mouse IgG1, κ
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Product Citations
IL-2 stimulated (18hrs) human peripheral blood lymphocytes stained with CD56 FITC and mAb 33 APC (top) or mouse IgG1,κ APC isotype control (bottom)
  • 33_158d_APC_top_04271
    IL-2 stimulated (18hrs) human peripheral blood lymphocytes stained with CD56 FITC and mAb 33 APC (top) or mouse IgG1,κ APC isotype control (bottom)
  • 33_158d_APC_zbottom_042710
    IL-2 stimulated (18hrs) human peripheral blood lymphocytes stained with CD56 FITC and mAb 33 APC (top) or mouse IgG1,κ APC isotype control (bottom)
See APC spectral data
Cat # Size Price Quantity Avail. Save
347007 25 tests 104€
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347008 100 tests 244€
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CD158 molecules, also known as KIRs (killer cell immunoglobulin-like receptors), are a family of transmembrane proteins with either two (KIR2D) or three (KIR3D) Ig-like extracellular domains. Some KIRs, with long cytoplasmic domains, contain ITIMs and possess inhibitory functions and others, with short cytoplasmic regions, lack ITIM and have activation functions. Fourteen polymorphic KIR genes have been reported in humans. KIR2DL4 (CD158d) is a unique receptor which has an ITIM in its cytoplasmic domain and a charged residue in the transmembrane domain. It possesses both inhibitory and activation functions. Two common alleles (10A and 9A) of KIR2DL4 have been reported. The 10A allele (with 10 adenines at the end of the transmembrane exon) receptor is expressed on CD56high NK subset, whereas its expression on CD56dim NK cells is inducible upon culture. The major 9A allele receptor is a secreted form. HLA-G is the ligand of CD158d.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Host Species
NK3.3 cells and KIR2DL4-Ig fusion protein
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

KIR2DL4 expression in NK cells is highly localized to the endosomes3. mAb 33 can be internalized by NK cells by endocytosis at 37°C3, and induces NK cell cytotoxicity1,2. Based on in-house observations, we suggest incubating for 45 - 60 minutes at room temperature with clone mAb 33 to observe better CD158d staining.

This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).

Application References

(PubMed link indicates BioLegend citation)
  1. Rajagopalan S, et al. 2001. J. Immunol. 167:1877. (FC)
  2. Goodridge JP, et al. 2003. J. Immunol. 171:1768. (FC)
  3. Rajagopalan S, et al. 2006. PLoS Biol. 4:e9.
Product Citations
  1. Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
  2. Brusilovsky M, et al. 2013. J Immunol. 191:5256. PubMed
AB_2249479 (BioLegend Cat. No. 347007)
AB_2130691 (BioLegend Cat. No. 347008)

Antigen Details

Contains two Ig-like extracellular domains, 45-50 kD

On CD56high NK cells and cultured CD56dim NK cells

Cell Type
NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Rajagopalan S, et al. 2006. PLoS Biol. 4:e9.
2. Goodridge JP, et al. 2007. Eur. J. Immunol. 37:199.

Gene ID
3805 View all products for this Gene ID
View information about CD158d on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 05.04.2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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