- QA17A17 (See other available formats)
- Regulatory Status
- Other Names
- PARP1, Poly (ADP-ribose) polymerase, PPOL, ARTD1, ADPRT, EC 2.4.2, ADP-Ribosyltransferase (NAD+;Poly (ADP-Ribose) Polymerase), Poly (ADP-Ribose) Polymerase Family, Member 1, DNA ADP-Ribosyltransferase PARP1, Poly[ADP-Ribose] Synthase 1
- Mouse IgG1, κ
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- Product Citations
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PARP (ADP-Ribose) Polymerase (PARP) is a 113 kD nuclear protein important for mediating the normal cellular response to DNA damage and repair in response to environmental stress. PARP catalyzes the transfer of ADP-ribose units from NAD⁺ to various nuclear proteins, including topoisomerases, histones, and PARP itself, by poly(ADP-ribosyl)ation. Following DNA damage, this protein is cleaved by ICE-like caspases, such as caspase-3 and -7. In humans, this PARP cleavage occurs between Asp214 and Gly215, yielding an 89 kD (from the carboxy-terminal catalytic domain) and a 24 kD fragment (from the amino-terminal DNA binding domain). PARP is an important regulator of cellular differentiation, proliferation, and tumor transformation, and PARP cleavage is considered a classical characteristic of cells undergoing apoptosis. Additionally, this enzyme may be the site of mutation in Fanconi anemia, and may contribute to the pathophysiology of type I diabetes.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Proprietary synthetic peptide
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC – Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Red Laser (633 nm)
- Application Notes
The QA17A17 antibody reacts with the 89 kD (cleaved at Asp214) fragment of human PARP1. It does not react with full-length PARP1.
Fixation and permeabilization using the True-Nuclear Buffer Set and Intracellular Staining Permeabilization Wash Buffer (10X) have also been confirmed to work in addition to the True-Phos™ Perm Buffer during in-house testing and development to detect cleaved PARP (Asp214).
AB_2910494 (BioLegend Cat. No. 669909)
AB_2910494 (BioLegend Cat. No. 669910)
- The large fragment of cleaved PARP is an 801 amino acid product with a predicted molecular weight of 89 kD
- Molecular “nick sensor”; base excision repair; catalyzes poly(ADP-ribosyl)ation of acceptor proteins involved in chromatin architecture; DNA metabolism; protein modification may enhance or repress transcription
- Component of a base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. Heterodimerizes with PARP2, interacts with PARP3, modified TATA-BP, YY1, Sp1, NF-B, p53 and others
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, DNA Repair/Replication, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
- Gene ID
- 142 View all products for this Gene ID
- View information about Cleaved PARP on UniProt.org
Related Pages & Pathways
Other FormatsView All Cleaved PARP Reagents Request Custom Conjugation
|PE anti-Cleaved PARP (Asp214) Recombinant Antibody||QA17A17||ICFC|
|Purified anti-Cleaved PARP (Asp214) Recombinant Antibody||QA17A17||WB,ICC,ICFC|
|PerCP/Cyanine5.5 anti-Cleaved PARP (Asp214) Recombinant Antibody||QA17A17||ICFC|
|APC anti-Cleaved PARP (Asp214) Recombinant Antibody||QA17A17||ICFC|
|PE/Cyanine7 anti-Cleaved PARP (Asp214) Recombinant Antibody||QA17A17||ICFC|
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