- IIB8 (See other available formats)
- Regulatory Status
- Other Names
- Softag 3
NeoClone Catalog# S0003
- Mouse IgG2a
- Ave. Rating
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- Product Citations
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Softag purification takes advantage of the unique properties of a class of monoclonal antibodies, termed polyol-responsive monoclonal antibodies which can be used in immunoaffinity purification. The advantage of the Polyol-Responsive mAb System is that the target protein is not denatured during purification, so it is suitable for the purification of labile, multi-subunit protein complexes. These protein complexes bind tightly to the polyol-responsive mAbs, but they are released under very gentle elution conditions. Additionally, the proteins are highly pure compared to other systems.Product Details
- Antibody Type
- Host Species
- 50% anti-RNA Polymerase antibody conjugated resin is supplied in 1X PBS and 0.09% NaN3. The volume specified for each catalog number indicates the volume of resin included.
- Storage & Handling
- Store at 4°C until use. Use at room temperature.
IP - Quality tested
- Recommended Usage
Resin binding capacity is greater than 0.3 mg/mL (based on 28 kD tagged protein). To elute, use TE, 0.7M NaCl, 30% propylene glycol.
- Application Notes
Previously item# S0003 from Neoclone.
This resin is also appropriate for use in identifying proteins associated with the recombinant protein tagged with Softag 3. Softag 3 is an immunoaffinity system appropriate for purifying tagged proteins from prokaryotic expression sources. The epitope (TQDPSRVG) is derived from a subunit of eukaryotic transcription factor TFIIB.
(PubMed link indicates BioLegend citation)
- Duellman SJ, et al. 2004. Protein Expr. Purif. 35:147.
- TFIIB is a 35 kD human transcription factor
- Biology Area
- Cell Biology
- Molecular Family
- Nuclear Markers
- Gene ID
- 2959 View all products for this Gene ID
- View information about RNA Polymerase TFIIB on UniProt.org
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