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Anti-CD49C (Integrin α3) Antibody (Previously Covance catalog# MMS-490R)

Pricing & Availability
Clone
P1B5 (See other available formats)
Regulatory Status
RUO
Other Names
GAP-B3, GAPB3, ILNEB, MSK18, VCA-2, VL3A, VLA3a, FRP-2, galactoprotein B3, VLA-3 subunit alpha, CD49 antigen-like family member C, antigen identified by monoclonal antibody J143, very late activation protein 3 receptor, alpha-3 subunit
Previously
Covance Catalog# MMS-490R
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P1B5_Ascites_CD49C_Antibody_1_032119_updated.png
IHC staining of anti-CD49C (Integrin α3) antibody (clone P1B5) on frozen human kidney tissue. The tissue was incubated with a 1:250 dilution of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • P1B5_Ascites_CD49C_Antibody_1_032119_updated.png
    IHC staining of anti-CD49C (Integrin α3) antibody (clone P1B5) on frozen human kidney tissue. The tissue was incubated with a 1:250 dilution of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • P1B5_Ascites_CD49C_Antibody_2_032119_updated.png
    HeLa cells were fixed with 4% PFA, permeabilized with Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) and stained with anti-CD49C (Integrin α3) antibody (clone P1B5, filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE (Cat. No. 405307).
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921601 100 µL 205€
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Description

CD49c is a 150 kD α integrin chain known as α3 integrin or VLA-3 α chain. It is a type I transmembrane glycoprotein which is proteolytically cleaved into two disulfide linked fragments of 125 kD and 30 kD. CD49c forms a heterodimer with integrin β1 (α3β1, CD49c/CD29, VLA-3) and is expressed by many types of adhesion cells, such as endothelial cells, epithelial cells, and dermal fibroblasts. Weak expression has been reported on leukocytes. VLA-3 plays a role in cell-cell and cell-matrix adhesion through binding Kalinin, collagen, laminin-1, laminin-5, entactin, and fibronectin.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The P1B5 monoclonal antibody was generated against human HT1080 cells.
Preparation
Ascites
Concentration
The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application

IHC-F - Quality tested
FC - Verified
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a dilution range of 1:250 - 1:500 is suggested. For flow cytometric staining, the suggested dilution of this reagent is between 1:250 - 1:500. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody recognizes the a3 subunit.

Application References

(PubMed link indicates BioLegend citation)
  1. Wayner EA, et al. 2007. Meths. Enzymol. In: Integrins. 426:117.
  2. Wayner EA, et al. 1993. J Cell Biol. 121:1141. (IP)
  3. Carter WG, et al. 1990. J Cell Biol. 110:1387. (IP)
  4. Wayner EA, Carter WG. 1987. J Cell Biol. 105:1873. (IP)
RRID
AB_2565403 (BioLegend Cat. No. 921601)

Antigen Details

Structure
CD49c is a 150 kD type I transmembrane glycoprotein
Distribution

Cellular distribution: Plasma membrane.
Tissue distribution: Endothelial cells, epithelial cells, and dermal fibroblast.

Biology Area
Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Adhesion Molecules
Gene ID
3675 View all products for this Gene ID
UniProt
View information about CD49c on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 03.21.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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