Anti-BACE1 (CO2 terminus) Antibody (Previously Covance catalog# PGP-624C)

Pricing & Availability
Clone
Poly8402 (See other available formats)
Regulatory Status
RUO
Other Names
Beta-Secretase 1, Asp 2, Memapsin-2, App Beta-Secretase, Aspartyl Protease 2, Beta-Site App Cleaving Enzyme 1, Beta-Secretase 1 Precursor Variant 1, Transmembrane Aspartic Proteinase Asp2, Membrane-Associated Aspartic Protease 2, Amyloid Beta A4
Previously
Covance Catalog# PGP-624C
Isotype
Guinea Pig Polyclonal
Ave. Rating
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Product Citations
publications
Purified_BACE1_Antibody_WB_030415
This image shows anti-BACE1 antibody GP45 used at a 1:1,000 dilution detecting BACE proteins which have been immunoprecipitated with anti-BACE1, B690, from an N2a cell lysate expressing human BACE. Photo courtesy of H. Xu, The Burnham Institute.
  • Purified_BACE1_Antibody_WB_030415
    This image shows anti-BACE1 antibody GP45 used at a 1:1,000 dilution detecting BACE proteins which have been immunoprecipitated with anti-BACE1, B690, from an N2a cell lysate expressing human BACE. Photo courtesy of H. Xu, The Burnham Institute.
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840201 100 µL 286,00€
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Description

Processing of the β-amyloid precursor protein (βAPP) by two endopeptidases, β and γ secretases generates the amyloidogenic peptide Aβ, a major factor in the etiology of Alzheimers disease. The beta secretase is a novel membrane bound aspartyl protease and cleaves the βAPP to generate the N-terminus of the Aβ domain. The membrane bound C-terminal fragment generated by the beta secretase cut is subsequently cleaved by the gamma secretase resulting in the generation and secretion of the Aβ.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Guinea Pig
Formulation
GP45 was raised in guinea pig against a peptide sequence CLRQQHDDFADDISLLK , the C-terminal 17 amino acids of the human BACE1 and recognizes the mature and immature forms of BACE.
Preparation
Serum
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application

WB - Quality tested
IP, ICC, IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution for this reagent is 1:1,000. It is recommended that the reagent be titrated for optimal performance for each application.

The optimal working dilution should be determined for each specific assay condition.
IP: 1:200
IF: 1:300

Application Notes

This antibody is effective in immunoblotting, immunoprecipitation (IP) and immunofluorescence (IF).

Clone also known as GP45.

Application References

(PubMed link indicates BioLegend citation)
  1. Yan R, et al. 2001. J Biol Chem. 276:36788-96.
Product Citations
  1. Liu L, et al. 2019. J Cell Biol. 218:644. PubMed
RRID
AB_2565446 (BioLegend Cat. No. 840201)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Gene ID
23621 View all products for this Gene ID
UniProt
View information about BACE1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 01.25.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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