Anti-BACE1 (CO2 terminus) Antibody (Previously Covance catalog# PRB-617C)

Pricing & Availability
Clone
Poly8401 (See other available formats)
Regulatory Status
RUO
Other Names
Beta-Secretase 1, Asp 2, Memapsin-2, App Beta-Secretase, Aspartyl Protease 2, Beta-Site App Cleaving Enzyme 1, Beta-Secretase 1 Precursor Variant 1, Transmembrane Aspartic Proteinase Asp2, Membrane-Associated Aspartic Protease 2, Amyloid Beta A4
Previously
Covance Catalog# PRB-617C
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Purified_BACE1_Antibody_IHC_1_030415
BACE subcellular localization in rat primary cortical neurons detected by BACE1 antibody B690 used at a 1:300 dilution. Photo courtesy of H. Xu, The Burnham Institute.
  • Purified_BACE1_Antibody_IHC_1_030415
    BACE subcellular localization in rat primary cortical neurons detected by BACE1 antibody B690 used at a 1:300 dilution. Photo courtesy of H. Xu, The Burnham Institute.
  • Purified_BACE1_Antibody_IHC_2_030415
    On the left, stable N2a cells overexpressing human BACE1 are immunprecipitated with B690 at a 1:250 dilution. The IP is detected on the blot with GP45 anti-BACE1. On the right, anti-BACE1 B690 used at 1:1,000 detects 2 bands on a standard Western blot of N2a cell lysates stably expressing human BACE1. Photo courtesy of H. Xu, The Burnham Institute.
Cat # Size Price Quantity Avail. Save
840101 100 µL 286,00€
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Description

Beta-secretase 1 (BACE1) is a novel membrane bound aspartyl protease that cleaves the β-amyloid precursor protein (βAPP) to generate the N-terminus of the Aβ domain. The membrane bound C-terminal fragment generated by the beta secretase cut is subsequently cleaved by the gamma secretase, resulting in the generation and secretion of the Aβ. The amyloidogenic peptide Aβ is a major factor in the etiology of Alzheimer’s disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
B690 was raised against a peptide sequence CLRQQHDDFADDISLLK , the C-terminal 17 amino acids of the human BACE1 and recognizes the mature and immature forms of BACE.
Preparation
Serum
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application

WB, ICC, IP

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
WB: 1:1,000
ICC: 1:300
IP: 1:200

Application Notes

This antibody is effective in immunoblotting, immunocytochemistry, (ICC) and immunoprecipitation (IP).

Processing of the β-amyloid precursor protein (βAPP) by two endopeptidases, β and γ secretases generates the amyloidogenic peptide Aβ, a major factor in the etiology of Alzheimers disease. The beta secretase is a novel membrane bound aspartyl protease which cleaves the β-APP to generate the N-terminus of the Aβ domain. The membrane bound C-terminal fragment generated by the beta secretase cut is subsequently cleaved by the gamma secretase, resulting in the generation and secretion of Aβ.

Clone also known as B690.


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Yan R, Han P, Miao H, Greengard P, Xu H. The transmembrane domain of the Alzheimer's beta-secretase (BACEdetermines its late Golgi localization and access to beta -amyloid precursor protein (APP) substrate. J Biol Chem 276(39):36788-36796, 2001. (WB, IP)
  2. Lysenko LV, et al. 2014. PLoS One.9:114521. (WB) PubMed
Product Citations
  1. Liu L, et al. 2019. J Cell Biol. 218:644. PubMed
  2. Lysenko L, et al. 2014. PLoS One. 9:e114521. PubMed
RRID
AB_2565445 (BioLegend Cat. No. 840101)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Gene ID
23621 View all products for this Gene ID
UniProt
View information about BACE1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 02.09.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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