Alexa Fluor® 647 anti-STAT1 Phospho (Ser727) Antibody

Pricing & Availability
Clone
A15158B (See other available formats)
Other Names
Signal transducer and activator of transcription 1 (STAT1), Transcription factor ISGF-3 components p91/p84
Isotype
Mouse IgG1, κ
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Product Citations
publications
A15158B_A647_PhosphoSer727_Antibody_1_032017
HeLa cells were stimulated with (filled histogram) or without (open histogram) nocodozole for 24 hours, fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then intracellularly stained with anti-STAT1 Phospho (Ser727) antibody (clone A15158B) Alexa Fluor™ 647.
  • A15158B_A647_PhosphoSer727_Antibody_1_032017
    HeLa cells were stimulated with (filled histogram) or without (open histogram) nocodozole for 24 hours, fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then intracellularly stained with anti-STAT1 Phospho (Ser727) antibody (clone A15158B) Alexa Fluor™ 647.
  • A15158B_A647_PhosphoSer727_Antibody_2_032017
    Human peripheral blood lymphocytes were stimulated with (filled histogram) or without (open histogram) Cell Activation Cocktail (without Brefeldin A) for 15 minutes, fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then intracellularly stained with anti-STAT1 Phospho (Ser727) antibody (clone A15158B) Alexa Fluor™ 647.
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
686411 25 tests 96€
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686412 100 tests 228€
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Description

STAT1, also known as signal transduction and activator of transcription 1, is a ubiquitously expressed cytoplasmic protein and is activated in response to cytokine signaling, including IFN-α, IFN-γ, EGF, PDGF, and IL-6. Upon activation, STAT1 is phosphorylated by receptor-associated kinases, translocates to the nucleus, and functions as a transcription factor. Two isoforms of STAT1, with apparent molecular weights of 88 and 91 kD, exist as a result of alternative RNA processing. STAT1 is involved in IFN-mediated immune responses, and STAT1-deficient mice are highly sensitive to bacterial and viral infections.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human STAT1 peptide phosphorylated at Ser 727.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
RRID
AB_2650785 (BioLegend Cat. No. 686411)
AB_2650786 (BioLegend Cat. No. 686412)

Antigen Details

Structure
750 amino acids, predicted molecular weight of 87 kD; contains a SH2 domain responsible for homodimerization or heterodimerization.
Distribution

Translocates to the nucleus when phosphorylated.

Function
Phosphorylated in response to cytokine signaling by receptor-associated kinases; translocates to the nucleus to act as a transcription factor. Mediates responses to type I (IFN-α/β) and II interferon (IFN-γ), EGF, PDGF, and IL-6.
Interaction
Forms a homodimer or heterodimers with other family members. Interacts with FAK, MCM3, MCM5, TRADD, BRCA1, KIT, IL-27R, IL-2Rβ, IL-2Rγ, IFNαβR, and c-Src.
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Phospho-Proteins, Nuclear Markers
Antigen References

1. Durbin JE, et al. 1996. Cell. 84:443.
2. Darnell JE Jr, et al. 1994. Science 264:1415.
3. Chen X, et al. 1998. Cell. 93:827.
4. Ramana CV, et al. 2000. Oncogene. 19:2619.

Gene ID
6772 View all products for this Gene ID
UniProt
View information about STAT1 Phospho on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 03.23.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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