Alexa Fluor® 647 anti-mouse IgD Antibody

Pricing & Availability
Clone
11-26c.2a (See other available formats)
Other Names
Immunoglobulin D
Isotype
Rat IgG2a, κ
Ave. Rating
1 reviews
Product Citations
publications
11-26Cdot2a_A647_042909.jpg
C57BL/6 splenocytes stained with 11-26c.2a Alexa Fluor® 647
  • 11-26Cdot2a_A647_042909.jpg
    C57BL/6 splenocytes stained with 11-26c.2a Alexa Fluor® 647
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
405707 25 µg 76€
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405708 100 µg 180€
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Description

Surface IgD is an important B cell differentiation marker.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® is a trademark of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3.

Application References

(PubMed link indicates BioLegend citation)
  1. Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
  2. Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
  3. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
  4. Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
  5. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  6. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
  7. Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
  8. Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
  9. Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed
Product Citations
  1. Chan T, et al. 2009. J Immunol. 183:3139. PubMed
  2. Vu T, et al. 2011. J Immunol. 187:5032. PubMed
  3. Gatto D, et al. 2011. J Immunol. 187:4621. PubMed
  4. Lahoud M, et al. 2011. J Immunol. 187:842. PubMed
  5. Kishi Y, et al. 2012. Proc Natl Acad Sci U S A. 109:7811. PubMed
  6. Lindner J, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  7. Dahlgren M, et al. 2015. J Immunol. 194:5187. PubMed
  8. Ogbe A, et al. 2015. J Biol Chem. 290: 20455-20465. PubMed
  9. Maity P, et al. 2015. Sci Signal. 8: ra93. PubMed
  10. Volkmann C, et al. 2016. EMBO J. 35: 2371 - 2381. PubMed
  11. Dotsey E, et al. 2017. Sci Rep. 7:42584. PubMed
  12. Jandl C, et al. 2017. Nat Commun. 10.1038/ncomms14647. PubMed
  13. Barwick BG, et al. 2018. Nat Commun. 9:1900. PubMed
  14. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
  15. Suan D et al. 2017. Immunity. 47(6):1142-1153 . PubMed
Publication Library
RRID
AB_893529 (BioLegend Cat. No. 405707)
AB_893528 (BioLegend Cat. No. 405708)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation
Cell Type
B cells
Biology Area
Immunology
Gene ID
380797 View all products for this Gene ID
UniProt
View information about IgD on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06.27.2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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