- 22F6 (See other available formats)
- Regulatory Status
- Other Names
- IKZF2, IKAROS family zinc finger 2, ANF1A2, ZNF1A2, ZNFN1A2
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
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Helios is a member of the Ikaros family of zinc finger transcription factors. It contains a C-terminal region composed of 2 zinc-finger domains that mediate dimerization between the family members. Helios was originally cloned from a mouse thymoma line. It is mainly expressed in peripheral T cells and thymocytes. It is found at high levels in a subpopulation of regulatory T cells. Helios plays an important role in T cell development and homeostasis. Overexpression of Helios profoundly alters αβ T cell differentiation and activation. It has been determined that silencing of Helios in B cells is critical for maintaining normal B cell function. Helios is also involved in tumor immunity.Product Details
- Mouse, Human
- Antibody Type
- Host Species
- Armenian Hamster
- Helios peptide (aa 51-107)
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol.
For flow cytometric staining, the suggested use of this reagent is 5 µl per 106 cells in 100 µl volume or 5 µl per 100 µl whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses
- Excitation Laser
Red Laser (633 nm)
- Application Notes
NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the True-Nuclear™ 10X Perm Buffer (Cat. No. 424403).
View more applications data for a different format of this clone in our Scientific Poster Library.
(PubMed link indicates BioLegend citation)
- Thornton AM, et al. 2010. J. Immunol. 184:1. PubMed
- Verhagen J and Wraith D. 2010. J. Immunol. 185:7129.
- Stone B, et al. 2012. Clin Immunol. 145:153. PubMed
- Vaeth M, et al. 2012. PNAS. 109:16258. PubMed
- Angin M, et al. 2014. PLoS One. 9:86920. PubMed
- Bedke T, et al. 2014. Immunol Cell Biol. PubMed
- Liu Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 307:177. PubMed
- Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed
- Product Citations
AB_10552902 (BioLegend Cat. No. 137208)
AB_10660750 (BioLegend Cat. No. 137218)
- A zinc finger nuclear transcription factor of the Ikaros family, containing 2 zinc-finger domains in C-terminal region.
Regulatory T cells (Tregs), conventional T cells, thymocytes.
- Plays an important role in T cell development and homeostasis, involved in tumor immunity.
- Cell Type
- T cells, Thymocytes, Tregs
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
1. Kelly CM, et al. 1998. Curr. Biol. 8:508.
2. Dovat S, et al. 2005. J. Immunol. 175:3508.
3. Cortes M, et al. 1999. Curr. Opin. Immunol. 11:167.
4. Cai Q, et al. 2009. J. Immunol. 183:2303.
5. Zhang Z, et al. 2007. Blood 109:2190.
6. Hahm K, et al. 1998. Genes Dev. 12:782.
- Gene ID
- 22779 View all products for this Gene ID 22807 View all products for this Gene ID
- View information about Helios on UniProt.org
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