Alexa Fluor® 647 anti-mouse CD34 Antibody

Pricing & Availability
SA376A4 (See other available formats)
Regulatory Status
Other Names
My10, gp105-120, mucosialin
Rat IgG2a, λ
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Product Citations
C57BL/6 mouse bone marrow cells were stained with anti-mouse Lineage Cocktail Pacific Blue™ and CD34 (clone SA376A4) Alexa Fluor® 647 (top) or rat IgG2a, κ Alexa Fluor® 647 isotype control (bottom). Data shown was gated on the total cell population.
  • SA376A4_A647_CD34_Antibody_1_070816
    C57BL/6 mouse bone marrow cells were stained with anti-mouse Lineage Cocktail Pacific Blue™ and CD34 (clone SA376A4) Alexa Fluor® 647 (top) or rat IgG2a, κ Alexa Fluor® 647 isotype control (bottom). Data shown was gated on the total cell population.
  • SA376A4_A647_CD34_Antibody_2_070816
Compare all formats See Alexa Fluor® 647 spectral data
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152205 25 µg 84€
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CD34, also known as gp105-120, is a highly glycosylated type I monomeric sialomucin-like glycophophoprotein with an approximate molecular weight of 105-120 kD, with two generated by alternative splicing. CD34 is expressed on hematopoietic progenitors, endothelial cells, brain, and testis. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands, and mediates the attachment of stem cells to the extracellular matrix or stromal cells. CD34 is phosphorylated on serine residues by PKC.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Mouse CD34 transfected cells.
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

This antibody (clone SA376A4) is useful for staining mouse CD34+ hematopoietic stem cell but it does not stain NIH-3T3 cells and the staining of Bend.3 cells is dimmer compared to other mouse CD34 antibodies, probably due to this antibody recognizes a different epitope than the other monoclonal antibodies.

Product Citations
  1. Chen Z, et al. 2020. Cell Death Dis. 11:180. PubMed
AB_2629649 (BioLegend Cat. No. 152205)

Antigen Details

Type I monomeric glycophophoprotein with an approximate molecular weight of 105-120 kD.

Hematopoietic progenitors, endothelial cells, brain and testis tissues.

Cell adhesion and homing.
L-selectin and E-selectin.
Cell Type
Endothelial cells, Hematopoietic stem and progenitors
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Garlanda C, et al. 1997. Eur J Cell Biol. 73:368-77.
2. Brown J, et al. 1991. Int Immunol. 3:175.
3. Suda J, et al. 1992. Blood. 79:2288-95.
4. Baumhueter S, et al. 1994. Blood. 84:2554.

Gene ID
12490 View all products for this Gene ID
View information about CD34 on

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Go To Top Version: 1    Revision Date: 07.20.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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