Alexa Fluor® 647 anti-mouse CD117 (c-Kit) Antibody

Pricing & Availability
Clone
2B8 (See other available formats)
Other Names
c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
a-2B8_Alx647_A_020708
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and Alexa Fluor® 647 rat IgG2a isotype control
  • a-2B8_Alx647_A_020708
    C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and Alexa Fluor® 647 rat IgG2a isotype control
  • b-2B8_Alx647_B_020708
    C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and 2B8 Alexa Fluor® 647
  • c-2B8_A647_CD117_Antibody_3_110419
    C57BL/6 mouse frozen testis section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of anti-mouse CD117 (clone 2B8) Alexa Fluor® 647 (red), and anti-mouse CD106 (clone 429 (MVCAM.A)) Alexa Fluor® 594 (green) overnight at 4°C. The image was captured by 10X objective.
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
105817 25 µg 68€
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105818 100 µg 156€
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Description

CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse bone marrow mast cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1 and immunohistochemistry of acetone fixed frozen sections2. The 2B8 antibody does not block c-Kit activity.

Application References

(PubMed link indicates BioLegend citation)
  1. Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
  2. Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
  3. Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
  4. Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
Product Citations
  1. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
  2. Hoshii T, et al. 2018. Cell. 172:1007. PubMed
  3. Barman PK, et al. 2019. J Immunol. 202:2720. PubMed
  4. Harhouri K, et al. 2010. Blood. 115:3843. PubMed
  5. Shade K, et al. 2015. J Exp Med. 212:457. PubMed
  6. Amouzegar A, et al. 2017. Stem Cells. 10.1002/stem.2611. PubMed
  7. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
  8. Barkaway A, et al. 2021. Immunity. . PubMed
RRID
AB_493475 (BioLegend Cat. No. 105817)
AB_493474 (BioLegend Cat. No. 105818)

Antigen Details

Structure
Ig superfamily, 145 kD
Distribution

Hematopoietic stem cells, AML, mast cells

Function
Growth factor receptor, tyrosine kinase
Ligand/Receptor
Stem Cell Factor (SCF)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.

Gene ID
16590 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 11.04.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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