- TRA-1-81 (See other available formats)
- Other Names
- Mouse IgM, κ
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- Product Citations
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The TRA-1-81 antibody reacts with a 200-240 kD cell surface antigen expressed by human embryonic stem cells (ESC), embryonic germ cells (EG), and embryonal carcinoma cells. The expression of TRA-1-81 on human ESC is downregulated upon differentiation. Further studies have shown that TRA-1-81 is a neuroaminidase-resistant carbohydrate epitope expressed on podocalyxin, a member of mucin-like glycoprotein with CD34 family. Podocalyxin is a type I tranmembrane sialoprotein with anti-adhesion property.Product Details
- Antibody Type
- Host Species
- Human embryonic carcinoma cell line 2102Ep cl.2A6
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
IF - Validated
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunofluorescence cell staining1, immunoprecipitation1, Western blotting1,2, ELISA1, and immunohistochemistry1 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.
(PubMed link indicates BioLegend citation)
- Andrews PW, et al. 1984. Hybridoma. 3:347.
- Schopperle WM and Dewolf WC. 2007. Stem Cells. 25:723.
- Product Citations
AB_1089245 (BioLegend Cat. No. 330705)
AB_1089242 (BioLegend Cat. No. 330706)
- Mucin-like protein, CD34 family member, 200-240 kD
- embryonic stem cells (ESC), embryonic germ cells (EG), embryonal carcinoma cells
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Cell Biology, Immunology, Stem Cells
- Antigen References
1. Andrews PW, et al. 1984. Hybridoma. 3:347.
2. Schopperle WM and Dewolf WC. 2007. Stem Cells. 25:723.
3. Takeda T, et al. 2000. Mol. Biol. Cell. 11:3219.
- Gene ID
- 5420 View all products for this Gene ID
- View information about TRA-1-81 on UniProt.org
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