Alexa Fluor® 647 anti-human Basophils (2D7) Antibody

Pricing & Availability
Basophil/2D7 (See other available formats)
Regulatory Status
Mouse IgG1, κ
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Human peripheral blood mononuclear cells surface stained with CCR3 PE and then intracellularly stained with Basophil/2D7 Alexa Fluor® 647
  • Basophilslash2D7_A647_030510
    Human peripheral blood mononuclear cells surface stained with CCR3 PE and then intracellularly stained with Basophil/2D7 Alexa Fluor® 647
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
346204 100 tests 236€
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The Basophil/2D7 mAb specifically recognizes the human basophil population. The antigen that this antibody binds is present in cell granules but its identity is still unknown.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Host Species
Lysates of purified basophils
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications for the relevant formats include: immunofluorescence staining7, and immunohistochemistry of acetone-fixed frozen sections1 and formalin-fixed paraffin-embedded sections1-6.

Application References

(PubMed link indicates BioLegend citation)
  1. Kepley CL, et al. 1995. J. Immunol. 154:6548. (IHC)
  2. Irani AM, et al. 1998. J. Allergy Clin. Immunol. 1998 101:354. (IHC)
  3. Agis H, et al. 2006. J. Clin. Pathol. 59:396. (IHC)
  4. Plager DA, et al. 2006. J. Allergy Clin. Immunol. 117:626. (IHC)
  5. Sperr WR, et al. 2001. Blood 98:2200. (IHC)
  6. Aichberger KJ, et al. 2006. Blood 108:3538. (IHC)
  7. Kepley CL, et al. 1998. J. Allergy Clin. Immun. 102:304. (IF)
AB_1967142 (BioLegend Cat. No. 346204)

Antigen Details



Cell Type
Biology Area
Immunology, Innate Immunity
Gene ID
View information about Basophil on

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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