Alexa Fluor® 647 anti-Histone H3 Phospho (Ser10) Antibody

Pricing & Availability
Clone
11D8 (See other available formats)
Other Names
Histone-H3, H3
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
11D8_AF647_Histone_H3_Phospho_Antibody_ICFC_100314
HeLa cells, which were treated with 20 µM of Nocodazole for 24 hours, were permeabilized with 70% ethanol and then stained with Histone H3 Phospho-Ser10 (clone 11D8) Alexa Fluor® 647 (filled histogram) or mouse IgG2b, κ Alexa Fluor® 647 isotype control (open histogram).
  • 11D8_AF647_Histone_H3_Phospho_Antibody_ICFC_100314
    HeLa cells, which were treated with 20 µM of Nocodazole for 24 hours, were permeabilized with 70% ethanol and then stained with Histone H3 Phospho-Ser10 (clone 11D8) Alexa Fluor® 647 (filled histogram) or mouse IgG2b, κ Alexa Fluor® 647 isotype control (open histogram).
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
650805 25 tests 140€
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650806 100 tests 308€
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Description

Histone H3 is phosphorylated at serine 10 during mitosis and is found to be involved in transcriptional activation, chromatin decondensation, and chromosome compaction during cell division, by the action of Aurora kinase and NIMA kinases.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Modified synthetic peptide conjugated to KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The histone H3 pS10 antibody recognizes phosphorylation of human H3 protein at Ser10 residue and has been shown to be useful for Western blotting.

RRID
AB_2564361 (BioLegend Cat. No. 650805)
AB_2564362 (BioLegend Cat. No. 650806)

Antigen Details

Structure
H3 is part of the nucleosome, comprised of an octameric complex with H2A, H2B, and H4 proteins.
Distribution
Nucleus
Function
H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones, therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication, and chromosomal stability.
Interaction
Two molecules of H3 form a heterotetramer with two molecules of H4.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, DNA Repair/Replication, Transcription Factors
Molecular Family
Phospho-Proteins
Antigen References

1. Choi HS, et al. 2005. J. Biol. Chem. 280:13545.
2. Goto H, et al. 2002. Genes Cells 7:11.
3. Garcia BA, et al. 2005. Biochemistry 44:13202.
4. Hans F, et al. 2001. Oncogene 20:3021.

Regulation
H3 is regulated by acetylation, methylation, citrullination, phosphorylation, and ubiquitination.
Gene ID
8290 View all products for this Gene ID
UniProt
View information about Histone H3 Phospho on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 10.10.2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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