Alexa Fluor® 594 anti-Nucleophosmin Antibody

Pricing & Availability
Clone
P87H10B9 (See other available formats)
Other Names
NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin
Isotype
Mouse IgG1, κ
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Product Citations
publications
P87H10B9_A594_Nucleophosmin_Antibody_030519
HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. The cells were intracellularly stained with 1 µg/ml Alexa Fluor® 594 anti-Nucleophosmin antibody (clone P87H10B9) overnight at 4°C. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • P87H10B9_A594_Nucleophosmin_Antibody_030519
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. The cells were intracellularly stained with 1 µg/ml Alexa Fluor® 594 anti-Nucleophosmin antibody (clone P87H10B9) overnight at 4°C. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
686803 25 µg 108€
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Description

Nucleophosmin is a member of the nucleophosmin/nucleoplasmin family that shuttles between the nucleus and cytoplasm. This protein regulates the stability and transcriptional activity of p53 and acts as a molecular chaperone. It preferentially binds to denatured proteins and has been shown to stimulate DNA polymerase activity and control the duplication of centrosomes. The chaperone activity of nucleophosmin is regulated by protein kinase CK2 and promotes the release of denatured substrates from nucleophosmin. It is modified by phosphorylation on Thr199. This protein has been shown to bind to RNA and DNA and interact with nucleolar proteins including nucleolin, protein P120, HIV-1 Rev protein, and hepatitis delta antigens.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant human nucleophosmin (2-265 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.2 - 1.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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RRID
AB_2617022 (BioLegend Cat. No. 686803)

Antigen Details

Structure
294 amino acids with a predicted molecular weight of 33 kD.
Distribution

Nucleolus.

Function
Involved in ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF.
Interaction
NSUN2, SENP3, hepatitis delta virus S-HDAg, HTLV1 Rex protein, methylated form of RPS10, APEX1, NEK2, ROCK2 and BRCA2, RPGR, CENPW, EIF2AK2/PKR, CEBPA (isoform 4), and DDX31.
Biology Area
Cell Biology
Molecular Family
Phospho-Proteins
Antigen References

1. Szebeni A, et al. 2003. J. Biol. Chem. 278:9107.
2. Colombo E, et al. 2002. Nat. Cell Biol. 4:529.
3. Chan PK, et al. 1986. J. Biol. Chem. 261:14335.

Gene ID
4869 View all products for this Gene ID
UniProt
View information about Nucleophosmin on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06.24.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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