Alexa Fluor® 594 anti-mouse IgG2a Antibody

Pricing & Availability
Clone
RMG2a-62 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin G2a
Isotype
Rat IgG
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Product Citations
publications
RMG2a-62_A594_IgG2a_Antibody_IHC-P_020218
Human frozen tonsil section was fixed with 4% paraformaldehyde (PFA) for ten minutes and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of anti-human CD20 (clone 2H7) Alexa Fluor® 488 (green) and 10 µg/ml of purified anti-human CD5 (clone L17F12) overnight at 4°C, followed by 2.5 µg/ml of anti-mouse IgG2a (clone RMG2a-62) Alexa Fluor® 594 (red) for 2 hours at room temperature. The image was captured by 10X objective.
  • RMG2a-62_A594_IgG2a_Antibody_IHC-P_020218
    Human frozen tonsil section was fixed with 4% paraformaldehyde (PFA) for ten minutes and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of anti-human CD20 (clone 2H7) Alexa Fluor® 488 (green) and 10 µg/ml of purified anti-human CD5 (clone L17F12) overnight at 4°C, followed by 2.5 µg/ml of anti-mouse IgG2a (clone RMG2a-62) Alexa Fluor® 594 (red) for 2 hours at room temperature. The image was captured by 10X objective.
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Check Availability Save
407119 25 µg 76,00€
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407120 100 µg 200,00€
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Description

The RMG2a-62 monoclonal antibody reacts with immunoglobulin G2a and G2c (IgG2a, IgG2c) and in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG2a-62 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application References

(PubMed link indicates BioLegend citation)
  1. Gavin AL, et al.2006. Science 314:1936.
  2. Podolin PL, et al. 2008. J. Immunol. 180:7989. (IP) PubMed
RRID
AB_2728430 (BioLegend Cat. No. 407119)
AB_2728431 (BioLegend Cat. No. 407120)

Related FAQs

IgG2a gene is deleted in some mouse strains, which ones are they?
IgG2a gene is deleted in C57Bl/6, C57Bl/ 10, SJL, and NOD mice. It is replaced with IgG2c gene instead.
Go To Top Version: 1    Revision Date: 02.02.2018

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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