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Alexa Fluor® 594 anti-E1 Ubiquitin Activating Enzyme Antibody

Pricing & Availability
Clone
2G2.3.5 (See other available formats)
Regulatory Status
RUO
Other Names
Ubiquitin-Activating Enzyme E1, Ubiquitin-Like Modifier Activating Enzyme 1, UBE1, A1S9T, GXP1, SMAX2, 2G.2.3.5, 2G2.3-5
Isotype
Mouse IgG1, κ
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Product Citations
publications
2G2.3.5_AF594_E1_Ubiquitin_Activating_Enzyme_Antibody_IF_082715
HeLa cells were fixed with 2% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Then, the cells were intracellularly stained with 2 µg/ml Alexa Fluor® 594 conjugated (red) E1 Ubiquitin Activating Enzyme Antibody (clone 2G2.3.5) in a blocking buffer overnight at 4°C. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • 2G2.3.5_AF594_E1_Ubiquitin_Activating_Enzyme_Antibody_IF_082715
    HeLa cells were fixed with 2% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Then, the cells were intracellularly stained with 2 µg/ml Alexa Fluor® 594 conjugated (red) E1 Ubiquitin Activating Enzyme Antibody (clone 2G2.3.5) in a blocking buffer overnight at 4°C. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
See Alexa Fluor® 594 spectral data
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679404 100 µg 324€
 
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Description

The proteasomal pathway of protein degradation involves two discrete steps: ubiquitination and degradation. Aside from proteasomal degradation, ubiquitination also plays an important role in transcriptional regulation, protein trafficking including endocytosis and lysosomal targeting, and the activation of kinases involved in signaling processes. Ubiquitination is carried out in three main steps: activation, conjugation, and ligation. E1 Ubiquitin Activating Enzyme (UBE1) initiates the cascade of conjugation by activating their respective modifiers. It is highly conserved in human, yeast, and plants. The gene for E1 Ubiquitin activating enzyme complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mixture of human and mouse E1 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application References

(PubMed link indicates BioLegend citation)
  1. Salvat C, et al. 2000. Eur. J. Biochem. 267:3712.
RRID
AB_2566181 (BioLegend Cat. No. 679404)

Antigen Details

Structure
1058 amino acids with a predicted molecular weight of approximately 118 kD.
Distribution

Ubiquitous, Eukaryotic cells.

Function
This protein catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation.
Interaction
GAN.
Biology Area
Cell Biology, DNA Repair/Replication, Signal Transduction, Transcription Factors, Ubiquitin/Protein Degradation
Molecular Family
Protein Modifiers
Antigen References

1. Xu W, et al. 2013. Curr. Pharm. Des. 18:3201.
2. Neutzner M, et al. 2012. Essays Biochem. 52:37.
3. Schulman BA, et al. 2009. Nat. Rev. Mol. Cell Biol. 10:319.
4. Salvat C, et al. 2000. Eur. J. Biochem. 267:3712.

Gene ID
7317 View all products for this Gene ID
UniProt
View information about E1 Ubiquitin Activating Enzyme on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 06.15.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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