Alexa Fluor® 594 anti-Cytoplasmic Dynein Intermediate Chain Antibody

Pricing & Availability
Clone
74.1 (See other available formats)
Other Names
Dynein light chain roadblock-type 1, Roadblock-1, ROBL/LC7-like 1, bithoraxoid-like protein, roadblock domain containing 1, dynein-associated protein Km23, cytoplasmic dynein light chain 2A, dynein light chain 2A, cytoplasmic
Isotype
Mouse IgG2b
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Product Citations
publications
74dot1_A594_Cytoplasmic_Dynein_Int_Chain_Antibody_ICC_051019.png
HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A-D) Alexa Fluor® 594 Anti- Cytoplasmic Dynein Intermediate Chain Antibody (Clone 74.1) or (E) Alexa Fluor® 594 Mouse IgG2b, κ Isotype Ctrl Antibody (Negative, Cat. No. 400362) overnight at 4°C. (B, D) Images are separate Alexa Fluor® 594 channel of images (A, C). Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time for all images (A-E) is 1/20 seconds. Concentrations for (A, B, E) is 5 µg/mL (1:100 dilution) and (C, D) is 2.5 µg/mL (1:200 dilution).
  • 74dot1_A594_Cytoplasmic_Dynein_Int_Chain_Antibody_ICC_051019.png
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A-D) Alexa Fluor® 594 Anti- Cytoplasmic Dynein Intermediate Chain Antibody (Clone 74.1) or (E) Alexa Fluor® 594 Mouse IgG2b, κ Isotype Ctrl Antibody (Negative, Cat. No. 400362) overnight at 4°C. (B, D) Images are separate Alexa Fluor® 594 channel of images (A, C). Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time for all images (A-E) is 1/20 seconds. Concentrations for (A, B, E) is 5 µg/mL (1:100 dilution) and (C, D) is 2.5 µg/mL (1:200 dilution).
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
904903 25 µg 108€
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904904 100 µg 252€
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Description

Dyneins are motor proteins that transport various cellular cargo along cytoskeletal microtubules in eukaryotic cells. Dynein has the same basic structures in all cell types and is composed of two or three distinct heavy chains (450 kD), three intermediate chains (70-125 kD), and at least four light chains (15-25 kD).  The cytoplasmic dynein intermediate chain is thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Vertebrates, Drosophila
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against purified bovine brain cytoplasmic dynein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

This antibody is effective in immunohistochemistry (IHC), immunoblotting and immunoprecipitation (IP).

*Predicted MW = 74 kD. This antibody is known not to react in squid and nematode.

Clone 74.1 reacts with all isoforms from both rat IC74 genes. The antigenic site is believed to be located in the conserved amino-terminal 60 amino acids. Clone 74.1 has been shown to inhibit the activity of cytoplasmic dynein.

Application References

(PubMed link indicates BioLegend citation)
  1. Miller MG, et al. 1999. Biol Reprod. 60:1047.
  2. Dillman JF, et al. 1996. Proc Natl Acad Sci USA. 93:141.
  3. Pfister KK, et al. 1996.J Biol Chem. 271:1687.
  4. Dillman JF, et al. 1994. J Cell Biol. 127:1671.
  5. Combs J, et al. 2006. PNAS. 103:14883.
  6. Chen JL, et al. 2005. J Cell Biol. 169:383. (IP) PubMed
  7. Burakov A, et al. 2008. Traffic. 9:472.
  8. Payne C, et al. 2003. J Cell Sci. 116:4727. (ICC) PubMed
  9. Semenova I, et al. 2014. Mol Biol Cell. 25:3119. (WB) PubMed

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Gene ID
83658 View all products for this Gene ID
UniProt
View information about Cytoplasmic Dynein Intermediate Chain on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 05.10.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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