Alexa Fluor® 488 anti-human CD326 (EpCAM) Antibody

Pricing & Availability
Clone
9C4 (See other available formats)
Regulatory Status
RUO
Other Names
Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, TROP1
Isotype
Mouse IgG2b, κ
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Product Citations
publications
9C4_Alx488_020708
Human colon carcinoma cell line HT29 stained with 9C4 Alexa Fluor® 488
  • 9C4_Alx488_020708
    Human colon carcinoma cell line HT29 stained with 9C4 Alexa Fluor® 488
See Alexa Fluor® 488 spectral data
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324209 25 tests 96,00€
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324210 100 tests 200,00€
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Description

CD326 is also known as Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, and TROP1. CD326 is a type I transmembrane protein containing six disulfide bridges and one THYRO domain. This cell surface glycosylated 40 kD protein is highly expressed in bone marrow, colon, lung, and most normal epithelial cells and is expressed on carcinomas of gastrointestinal origin. Recently, it has been reported that CD326 expression occurs during the early steps of erythrogenesis. CD326 functions as a homotypic calcium-independent cell adhesion molecule and is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation. CD326 antigen is an immunotherapeutic target for the treatment of human carcinomas.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
DU.4475 breast carcinoma
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the revelant formats) include: immunofluorescence and immunohistochemistry3.

Application References

(PubMed link indicates BioLegend citation)
  1. Lammers R, et al. 2002. Exp. Hematol. 30:537.
  2. Schultz LD, et al. 2010. P. Natl. Acad. Sci. USA 107:13022. PubMed
  3. Human Protein Atlas http://www.proteinatlas.org/ENSG00000119888/antibody (IHC)
Product Citations
  1. Cai X, et al. 2017. Neoplasia. 10.1016/j.neo.2017.03.005. PubMed
  2. Gell JJ, et al. 2018. Stem Cell Res. 27:46. PubMed
  3. Hu H et al. 2018. Cell. 175(6):1591-1606 . PubMed
  4. Chen D et al. 2018. Cell reports. 25(13):3591-3602 . PubMed
  5. Chen D, et al. 2017. Biol Reprod. 97:850. PubMed
  6. Forbes TA, et al. 2018. Am J Hum Genet. 102:816. PubMed
  7. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  8. Pal D et al. 2017. eLife. 6 pii: e21615. PubMed
  9. Gerdur ísberg ó, et al. 2019. Sci Rep. 9:14843. PubMed
  10. Chen D, et al. 2019. Cell Rep. 29:4568. PubMed
  11. Calandrini C, et al. 2020. Nat Commun. 11:1310. PubMed
  12. Vanslambrouck JM, et al. 2019. J Am Soc Nephrol. 30:1811. PubMed
  13. Hong HK, et al. 2019. Oncol Rep. 42:2029. PubMed
  14. Horwitz K, et al. 2008. Proc Natl Acad Sci U S A. 105:5774. PubMed
  15. Morris K, et al. 2014. PLoS One. 9:98656. PubMed
  16. Noort V, et al. 2014. Cancer Res. 74:5690. PubMed
  17. Cai X, et al. 2014. PLoS One. 9:108942. PubMed
  18. Catenacci D, et al. 2015. Gastroenterology. 149: 1794-1803.e4. PubMed
  19. Morsing M, et al. 2020. Breast Cancer Res. 0.9875. PubMed
  20. Zabala M, et al. 2020. Cell Stem Cell. 27(2):284-299.e8. PubMed
  21. Yu L, et al. 2020. Cell Stem Cell. 28(3):550-567.e12. PubMed
  22. Howden SE, et al. 2020. Cell Stem Cell. 28(4):671-684.e6. PubMed
  23. Mourcin F, et al. 2021. Immunity. . PubMed
  24. Cardenas JJ, et al. 2020. Methods Enzymol. 415:631. PubMed
  25. Pyo DH, et al. 2020. Cancer Biol Ther. 495:21. PubMed
  26. Chitiashvili T, et al. 2020. Nat Cell Biol. 22:1436. PubMed
  27. Safaric Tepes P, et al. 2021. Elife. 10: . PubMed
RRID
AB_756083 (BioLegend Cat. No. 324209)
AB_756084 (BioLegend Cat. No. 324210)

Antigen Details

Structure
Type I transmembrane protein, contains six disulfide bridges, one THYRO domain, approximate molecular weight 40 kD.
Distribution

Highly expressed in bone marrow, colon, lung, and most normal epithelial cells. Also highly expressed on carcinomas of gastrointestinal origin. Expressed during early erythrogenesis.

Function
Homotypic calcium-independent cell adhesion. CD326 is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation.
Modification
Glycosylated.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Strnad J, et al. 1989. Cancer Res. 49:314.
2. Munz M, et al. 2004. Oncogene 23:5748.
3. Rao CG, et al. 2005. Int. J. Oncol. 27:49.

Gene ID
4072 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 11.03.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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