Product: FITC anti-human CD244 (2B4) |
Catalog No.: 329505 |
Katherine Nicholas, PhD Candidate Vanderbilt University |
1uL 2B4-FITC Works Great! | |||
Overall: | Product Quality: | Ease of Use: | |
1uL of 2B4-FITC worked well to detect 2B4+ lymphocytes. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Human PBMC |
Brief Protocol: | Cyropreserved human PBMC were thawed, washed twice in PBS, and stained with a cocktail of antibodies including 2B4-FITC. Cells were incubated with antibodies for 15 minutes at room temperature and 1uL 2B4-FITC was used to stain 1 million cells in 200uL PBS. |
Results Summary: | 1uL 2B4-FITC worked great to detect 2B4+ lymphocytes ex vivo. |
Additional Notes: |
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