Product: LEGEND MAX™ Mouse IFN-β ELISA Kit |
Catalog No.: 439407 |
Maria Carmela Speranza, postdoc BWH/HMS |
ELISA Mouse IFN-β | |||
Overall: | Product Quality: | Ease of Use: | |
Easy and fast ELISA assay. |
Experimental Design |
Application: | ELISA |
Cells used: | Tumor cell supernatant |
Brief Protocol: | Follow the datasheet - it will tell everything you need to know. - The plate is pre-coated, therefore just wash the plate 3 time before use. - Incubate 2h RT 200rpm with your sample (cell supernant) and with you standard dilutions. - Wash 4 times - Incubate 2h RT 200rpm with 100ul of mouse IFNbeta - Wash 4 times - Incubate 30min RT 200rpm with 100ul of Avidin-HRP - Wash 5 times - Incubate 30min RT in the dark with 100ul of Substrate solution F - Add 100ul of Stop solution - Read at 450nm |
Results Summary: | Perfect standard curve, good duplicates. |
Additional Notes: |
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