Product: PE/Cyanine7 anti-mouse IL-10

Catalog No.: 505026

J Lykken, Dr. Duke University

Labels IL-10+ Mouse Splenocytes
Overall:	Product Quality:	Ease of Use:
Labels IL-10+ mouse splenocytes, although it is not as bright as other antibodies.

Experimental Design

Application:	Flow Cytometry
Cells used:	Mouse Splenocytes
Brief Protocol:	Isolate splenocytes from mice. Wash cells once in FACS buffer (PBS plus 1% BSA or serum). Block Fc receptors and stain for viability in PBS for 20 minutes on ice. Wash cells once in FACS buffer (PBS plus 1% BSA or serum). Permeabilize cells. Stain with isotype control or IL-10 PECyanine7 antibody (2 ug/mL in Perm buffer, 1:100 dilution, 100 uL per million cells) for 20 minutes on ice. Add Perm buffer to top of tube and incubate on ice for 10 minutes. After washing, resuspend cells in 100-250 uL FACS buffer of 1.5% paraformaldehyde.
Results Summary:	Labels IL-10+ mouse splenocytes, although it is not as bright as other antibodies.
Additional Notes:	This is the optimal clone to stain IL-10, however IL-10 PE (505008) is much brighter.

Data Image Click on the image for full size data.

Publication Status This data has been published. Pubmed

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