Application: |
Whole mount fluorescent immuno and cryo section immune |
Cells used: |
Mouse embryo (E11.5) and embryonic kidney (E15.5) |
Brief Protocol: |
No antigen retrieval necessary Block 20 min-1 hour donkey serum (section) or sheep serum (whole mount) O/N primary incubation for whole mount (1:250 anti-Tuj1) or 1-2 hr incubation for section (1:1000) O/N secondary incubation for whole mount (1:250 Alexa Fluor 488 donkey anti-mouse IgG2a ) or 1 hr incubation for section (1:1000 ) Whole mount tissue cleared with iDISCO+
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Results Summary: |
Excellent, specific staining with no background. Antibody holds up to iDISCO+ tissue clearing and gave great signal on light sheet microscope. |
Additional Notes: |
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