Application: |
Flow cytometry |
Cells used: |
Human peripheral blood mononuclear cells (PBMCs) |
Brief Protocol: |
<1x10^6 PBMCs per 100 ul of PBS+1mM EDTA+ 2% FBS were incubated with 2.5 ul of the antibody for 20-30 min at 4 C.
1-2 ml of PBS+1mM EDTA+ 2% FBS were then added and cells centrifuged for 5 min at 1000 g. Most of the supernatant was removed and then 200 ul of cold PBS+1mM EDTA+ 2% FBS were added for flow cytometry analysis. |
Results Summary: |
A high proportion of monocytes within the PBMCS showed expression of CCR2 as expected. |
Additional Notes: |
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