Product: PE anti-human Ganglioside GD2 |
Catalog No.: 357303 |
Sarah Pierce, Undergraduate WIBR |
GD2 (PE anti-human Ganglioside) | |||
Overall: | Product Quality: | Ease of Use: | |
GD2 as a surface marker on mesenchymal cell lines. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | SUM159 |
Brief Protocol: | Cells were trypsinized, neutralized with DMEM 10%, and re-suspended in ice-cold PBS with 2% IFS at 1x10^6 cells per 100 uL. The cells were incubated on ice with 1:100 antibody for 30 min, washed with 9 times the original volume of ice-cold PBS- with 2% IFS, and re-suspended in 500 uL ice-cold PBS- with 2% IFS. |
Results Summary: | 10.3% of the cells were GD2 high. |
Additional Notes: |
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Technical Service Notes |
Trypsinization can affect epitopes as it is enzyme-based. This can make it difficult for antibodies to recognize their targets. |
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