Product: APC/Fire™ 750 anti-mouse/human CD44

Catalog No.: 103062

Priya Devarajan, Postdoctoral Associate University of Massachusetts Medical School

APC/Fire™ 750 very stable with fixation unlike APC Fluor 780
Overall:	Product Quality:	Ease of Use:
CD44 APC/Fire™ 750 works very well, without disintegrating on fixation with 2%PFA or with the commerical fix/perm for Foxp3 staining, though not super bright.

Experimental Design

Application:	Flow cytometry phenotyping of mouse spleen and LNs
Cells used:	Mouse lymphocytes
Brief Protocol:	Cells were stained for 30minutes with CD44 APC Fire 750 at a dilution of 1:100 in 50uL of FACS buffer. The cells were then washed 2x and fixed with 2% PFA for 30mins or with ebioscience fixation buffer (for Foxp3 staining) overnight. The cells were then washed and run on a flow cytometer or were permeabilized the next day for Foxp3 staining after which they were washed and run on a flow cytometer.
Results Summary:	Both dilutions of 1:100 and 1:50 worked very well. There was no disintergration of APC Fire 750 into APC. This really helped use the same flurochrome for both surface and transcription factor panels. I wasn't able to do this before when I used CD44 APC efluor 780.
Additional Notes:

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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