Product: FITC anti-human CD54 |
Catalog No.: 353107 |
Ahmad Al-Attar, Dr. Univeristy of Kentucky |
Good Marker for Monocyte Activation (FITC anti-human CD54) | |||
Overall: | Product Quality: | Ease of Use: | |
This antibody will stain activated monocytes clearly! |
Experimental Design |
Application: | Flow Cytometry |
Cells used: | THP-1 cells |
Brief Protocol: | Stimulate cells with PMA/IL-4 and test for activation by staining them (and untreated controls). Wash with FACS buffer. Add antibody (5uL per million cells) and incubate for 30 minutes. Wash, and read on FL-1 channel. |
Results Summary: | The grey histogram shows untreated cells; the black-line open histogram are cells treated with PMA/IL-4. |
Additional Notes: | Great antibody, easy to use. Good price! |
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