Product: Alexa Fluor® 488 anti-mouse CD3ε |
Catalog No.: 100321 |
Adam Guess, SR Research Associate The Research Institute at Nationwide Children's Hospital |
Staining mouse splenocytes with anti-CD3ε Alexa Fluor® 488. | |||
Overall: | Product Quality: | Ease of Use: | |
Anti-CD3 Alexa Fluor® 488 works well on mouse splenocytes. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Mouse splenocytes |
Brief Protocol: | ACK-lysed mouse splenocytes were preincubated with 1ug anti-mouse FcBlock for 5 minutes and stained with a 1:100 dilution of anti-CD3 Alexa Fluor® 488 for 15 minutes at room temperature in 100uL FACS buffer (PBS w/2% FBS and 0.09% sodium azide). Cells were washed twice with 2mL FACS buffer and analyzed on a BD LSR II. |
Results Summary: | CD3 staining was excellent (filled histogram), as compared to unstained cells (dotted line). |
Additional Notes: |
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